• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

肉食植物茅膏菜分泌的消化酶

Digestive enzymes secreted by the carnivorous plant Nepenthes macferlanei L.

机构信息

Department of Biochemistry, University of Malaya, Kuala Lumpur, Malaysia.

出版信息

Planta. 1974 Mar;119(1):39-46. doi: 10.1007/BF00390820.

DOI:10.1007/BF00390820
PMID:24442407
Abstract

At least two proteases are present in the secretion of the pitchers of Nepenthes macferlanei, a major one with an estimated molecular weight of 59000 and a minor one of 21000. The specificity of the major enzyme, nepenthesin, was broader than previously reported, and strikingly similar to that of pepsin. Lipase activity was also demonstrated, while no amylase activity was present.

摘要

在大猪笼草的分泌液中至少存在两种蛋白酶,其中一种主要蛋白酶的分子量估计为 59000,另一种次要蛋白酶的分子量为 21000。主要酶(猪笼草凝乳酶)的特异性比之前报道的更广泛,与胃蛋白酶惊人地相似。还证明了脂肪酶的活性,而不存在淀粉酶的活性。

相似文献

1
Digestive enzymes secreted by the carnivorous plant Nepenthes macferlanei L.肉食植物茅膏菜分泌的消化酶
Planta. 1974 Mar;119(1):39-46. doi: 10.1007/BF00390820.
2
Enzyme activities in two sister-species of carnivorous pitcher plants (Nepenthes) with contrasting nutrient sequestration strategies.两种具有不同营养隔离策略的肉食性猪笼草(猪笼草属)姐妹种中的酶活性。
Plant Physiol Biochem. 2021 Apr;161:113-121. doi: 10.1016/j.plaphy.2021.01.049. Epub 2021 Feb 5.
3
Nepenthesin protease activity indicates digestive fluid dynamics in carnivorous nepenthes plants.猪笼草蛋白酶活性表明食虫猪笼草植物中消化液的动态变化。
PLoS One. 2015 Mar 9;10(3):e0118853. doi: 10.1371/journal.pone.0118853. eCollection 2015.
4
Discovery of digestive enzymes in carnivorous plants with focus on proteases.食肉植物中消化酶的发现,重点是蛋白酶。
PeerJ. 2018 Jun 5;6:e4914. doi: 10.7717/peerj.4914. eCollection 2018.
5
Proteome analysis of digestive fluids in Nepenthes pitchers.猪笼草捕虫笼消化液的蛋白质组分析。
Ann Bot. 2016 Mar;117(3):479-95. doi: 10.1093/aob/mcw001.
6
Expression and characterization of plant aspartic protease nepenthesin-1 from Nepenthes gracilis.来自细叶猪笼草的植物天冬氨酸蛋白酶猪笼草蛋白酶-1的表达与特性分析
Protein Expr Purif. 2014 Mar;95:121-8. doi: 10.1016/j.pep.2013.12.005. Epub 2013 Dec 21.
7
Slow food: insect prey and chitin induce phytohormone accumulation and gene expression in carnivorous Nepenthes plants.慢食:昆虫猎物和几丁质诱导食肉猪笼草植物中植物激素积累和基因表达。
Ann Bot. 2016 Aug;118(2):369-75. doi: 10.1093/aob/mcw110. Epub 2016 Jun 20.
8
Recombinant Nepenthesin II for Hydrogen/Deuterium Exchange Mass Spectrometry.用于氢/氘交换质谱分析的重组猪笼草蛋白酶II
Anal Chem. 2015 Jul 7;87(13):6681-7. doi: 10.1021/acs.analchem.5b00831. Epub 2015 Jun 8.
9
Aspartic proteinases are expressed in pitchers of the carnivorous plant Nepenthes alata Blanco.天冬氨酸蛋白酶在食虫植物菲律宾猪笼草(Nepenthes alata Blanco)的捕虫笼中表达。
Planta. 2002 Mar;214(5):661-7. doi: 10.1007/s004250100665. Epub 2001 Oct 16.
10
Carnivorous Nutrition in Pitcher Plants (Nepenthes spp.) via an Unusual Complement of Endogenous Enzymes.猪笼草属植物(猪笼草属物种)通过独特的内源性酶组合实现食肉营养。
J Proteome Res. 2016 Sep 2;15(9):3108-17. doi: 10.1021/acs.jproteome.6b00224. Epub 2016 Aug 1.

引用本文的文献

1
How the diversity in digestion in carnivorous plants may have evolved.食肉植物消化方式的多样性是如何进化而来的。
New Phytol. 2025 Sep;247(6):2581-2590. doi: 10.1111/nph.70229. Epub 2025 May 28.
2
Diverse activities and biochemical properties of amylase and proteases from six freshwater fish species.六种淡水鱼的淀粉酶和蛋白酶的多种活性和生化特性。
Sci Rep. 2021 Mar 11;11(1):5727. doi: 10.1038/s41598-021-85258-7.
3
Genome-wide identification, evolution and expression analysis of the aspartic protease gene family during rapid growth of moso bamboo (Phyllostachys edulis) shoots.

本文引用的文献

1
[Purification of the proteinase from Nepenthes pitcher secretion].[从猪笼草捕虫笼分泌液中纯化蛋白酶]
Planta. 1967 Sep;76(3):238-41. doi: 10.1007/BF00409815.
2
Notes on sugar determination.糖分测定笔记。
J Biol Chem. 1952 Mar;195(1):19-23.
3
Amino-acid sequence of horse heart cytochrome c.马心脏细胞色素c的氨基酸序列。
毛竹(Phyllostachys edulis)笋快速生长过程中天冬氨酸蛋白酶基因家族的全基因组鉴定、进化及表达分析
BMC Genomics. 2021 Jan 10;22(1):45. doi: 10.1186/s12864-020-07290-7.
4
Pitchers of Nepenthes khasiana express several digestive-enzyme encoding genes, harbor mostly fungi and probably evolved through changes in the expression of leaf polarity genes.基纳巴卢猪笼草的瓶状叶中表达了几种消化酶编码基因,这些基因主要定殖有真菌,并且可能是通过叶片极性基因表达的变化而进化来的。
BMC Plant Biol. 2020 Nov 17;20(1):524. doi: 10.1186/s12870-020-02663-2.
5
Molecular Properties and New Potentials of Plant Nepenthesins.植物猪笼草蛋白酶的分子特性与新潜力
Plants (Basel). 2020 Apr 29;9(5):570. doi: 10.3390/plants9050570.
6
Protein replenishment in pitcher fluids of Nepenthes × ventrata revealed by quantitative proteomics (SWATH-MS) informed by transcriptomics.定量蛋白质组学(SWATH-MS)结合转录组学揭示的猪笼草 × 猪笼草 pitchers 液体中的蛋白质补充。
J Plant Res. 2019 Sep;132(5):681-694. doi: 10.1007/s10265-019-01130-w. Epub 2019 Aug 17.
7
Discovery of digestive enzymes in carnivorous plants with focus on proteases.食肉植物中消化酶的发现,重点是蛋白酶。
PeerJ. 2018 Jun 5;6:e4914. doi: 10.7717/peerj.4914. eCollection 2018.
8
Effects of prey, pitcher age, and microbes on acid phosphatase activity in fluid from pitchers of Sarracenia purpurea (Sarraceniaceae).猎物、捕虫笼年龄和微生物对紫瓶子草(瓶子草科)捕虫笼内液体中酸性磷酸酶活性的影响。
PLoS One. 2017 Jul 18;12(7):e0181252. doi: 10.1371/journal.pone.0181252. eCollection 2017.
9
Addressing proteolytic efficiency in enzymatic degradation therapy for celiac disease.解决乳糜泻酶解治疗中蛋白水解效率的问题。
Sci Rep. 2016 Aug 2;6:30980. doi: 10.1038/srep30980.
10
Proteome analysis of digestive fluids in Nepenthes pitchers.猪笼草捕虫笼消化液的蛋白质组分析。
Ann Bot. 2016 Mar;117(3):479-95. doi: 10.1093/aob/mcw001.
Nature. 1961 Dec 23;192:1125-7. doi: 10.1038/1921125a0.
4
Peptide separation by two-dimensional chromatography and electrophoresis.通过二维色谱法和电泳法进行肽分离。
J Biol Chem. 1959 Nov;234:2897-900.
5
A modified ninhydrin reagent for the photometric determination of amino acids and related compounds.一种用于光度法测定氨基酸及相关化合物的改良茚三酮试剂。
J Biol Chem. 1954 Dec;211(2):907-13.
6
Determination of proteins in the presence of phenols and pectins.在存在酚类和果胶的情况下蛋白质的测定
Anal Biochem. 1969 Jun;29(3):535-9. doi: 10.1016/0003-2697(69)90339-x.
7
Acid protease in Nepenthes. II. Study on the specificity of nepenthesin.猪笼草中的酸性蛋白酶。II. 猪笼草蛋白酶特异性的研究。
J Biochem. 1969 Oct;66(4):431-9. doi: 10.1093/oxfordjournals.jbchem.a129166.
8
The structure of pepstatin.胃蛋白酶抑制剂的结构。
J Antibiot (Tokyo). 1970 May;23(5):263-5. doi: 10.7164/antibiotics.23.263.
9
An aspartic acid residue at the active site of pepsin. The isolation and sequence of the heptapeptide.胃蛋白酶活性位点的一个天冬氨酸残基。七肽的分离与序列分析。
Biochem J. 1969 Jun;113(2):377-86. doi: 10.1042/bj1130377.