Agricultural Research Service, US Department of Agriculture, Snake River Conservation Research Center, 83341, Kimberly, ID, USA.
Photosynth Res. 1986 Jan;8(2):123-32. doi: 10.1007/BF00035243.
Measurement of the changes in CO2 uptake by single leaves following the abrupt onset of darkness were made on sugarbeets (Beta vulgaris L.) and (Phaseolus vulgaris L.) The shape of the CO2 dark response curve was analyzed with respect to the reaction kinetics of CO2, RuP2 and RuP2 carboxylase. It was concluded that the net uptake of CO2 in the dark from a 1% O2 atmosphere can be approximately related to the pool size of the RuP2 substrate in the chloroplasts of C3 plants. This information was combined with CO2 levels and decay rates of the response curves to infer changes in carboxylase activity. Preliminary data are presented showing the relative concentration changes in RuP2 as light intensity decreases and as water stress increases. The method may prove useful in studies of plant response to environmental stresses.
对糖甜菜（Beta vulgaris L.）和菜豆（Phaseolus vulgaris L.）的单叶进行了研究，测量了黑暗突然开始后 CO2 摄取量的变化。根据 CO2、RuP2 和 RuP2 羧化酶的反应动力学，分析了 CO2 暗响应曲线的形状。得出的结论是，在黑暗中从 1%O2 大气中净吸收 CO2 可以与 C3 植物叶绿体中 RuP2 底物的池大小大致相关。该信息与 CO2 水平和响应曲线的衰减率相结合，以推断羧化酶活性的变化。初步数据表明，随着光强度的降低和水分胁迫的增加，RuP2 的相对浓度变化。该方法在研究植物对环境胁迫的反应时可能会很有用。
