Stephenson E C, Mahowald A P
Department of Biology, University of Rochester, New York 14627.
Dev Biol. 1987 Nov;124(1):1-8. doi: 10.1016/0012-1606(87)90453-2.
We have isolated molecular clones encoding RNAs which appear to be transcribed predominantly or exclusively during oogenesis in Drosophila melanogaster. These clones were isolated by virtue of their homology to polyadenylated RNAs present in very early embryos and absent from older embryos. An analysis of the developmental expression of three cloned genes is presented. The genomic position of one of these clones coincides closely with the position of the maternal effect lethal gene swallow, providing strong evidence that we have cloned this locus.
我们已经分离出了编码RNA的分子克隆，这些RNA似乎主要或仅在黑腹果蝇的卵子发生过程中被转录。这些克隆是通过与早期胚胎中存在而较老胚胎中不存在的多聚腺苷酸化RNA的同源性分离出来的。本文给出了对三个克隆基因发育表达的分析。其中一个克隆的基因组位置与母体效应致死基因swallow的位置紧密重合，这有力地证明我们已经克隆到了这个基因座。
