Kolchinsky A M, Vashakidze R P, Korochkin L I
Cell Differ. 1986 Mar;18(2):145-9. doi: 10.1016/0045-6039(86)90009-6.
DNA fragments actively transcribed at different developmental stages of Drosophila melanogaster were cloned. In situ hybridization was used to study their distribution in the polytene chromosomes. The clones were dot-hybridized to poly(A)+ RNA isolated at different developmental stages. Fifty-four of 67 clones were unique, 37 of them were localized. An irregular distribution of the cloned sequences was revealed. Twenty-three clones are localized in chromosome 3, six clones in the X-chromosome, and only eight in chromosome 2. There are transcriptionally active regions where, on a relatively small area of the chromosomes, a number of clones are localized (sections 18-19, 69-71, 82-85, 95-97). RNAs transcribed from the cloned sequences code for about 0.005 to 0.2% of the total poly(A)+ RNA.
克隆了在黑腹果蝇不同发育阶段活跃转录的DNA片段。采用原位杂交技术研究它们在多线染色体中的分布。将这些克隆与在不同发育阶段分离的聚腺苷酸加尾RNA（poly(A)+ RNA）进行点杂交。67个克隆中有54个是独特的，其中37个已定位。揭示了克隆序列的不规则分布。23个克隆定位于3号染色体，6个克隆定位于X染色体，而只有8个定位于2号染色体。存在转录活跃区域，在染色体相对较小的区域内定位有许多克隆（第18 - 19节、69 - 71节、82 - 85节、95 - 97节）。从克隆序列转录的RNA约占总聚腺苷酸加尾RNA的0.005%至0.2%。
