Goldstein E S, Vincent W S, Schultz K A
Biochim Biophys Acta. 1986 Aug 22;867(4):209-19. doi: 10.1016/0167-4781(86)90036-9.
A lambda recombinant DNA library containing Drosophila melanogaster nuclear DNA inserts was screened with cDNA made from oocyte and gastrula poly(A)+ RNA. 124 clones were isolated which represented sequences complementary to a distribution of abundancies of their RNAs. The clone set was then used as probes to identify those whose RNA abundancies changed during embryonic development. The vast majority of clones showed little difference during development. Four different clones were identified whose poly(A)+ RNAs were quantitatively regulated; two were oocyte-specific, and two were embryonic-specific. 44 clones were chosen for in situ hybridization to salivary gland polytene chromosomes. The location and distribution of their sites are described. A class of clones, identified by in situ hybridization to the nucleolus, is further described. These clones contain a scrambled array of ribosomal intervening sequences.
用从卵母细胞和原肠胚多聚腺苷酸（poly(A)）+ RNA制备的cDNA筛选了一个含有黑腹果蝇核DNA插入片段的λ重组DNA文库。分离出124个克隆，它们代表与其RNA丰度分布互补的序列。然后将该克隆集用作探针，以鉴定那些RNA丰度在胚胎发育过程中发生变化的克隆。绝大多数克隆在发育过程中差异不大。鉴定出四个不同的克隆，其多聚腺苷酸（poly(A)）+ RNA受到定量调节；两个是卵母细胞特异性的，两个是胚胎特异性的。选择44个克隆用于与唾液腺多线染色体进行原位杂交。描述了它们位点的位置和分布。进一步描述了一类通过与核仁进行原位杂交鉴定的克隆。这些克隆包含一系列混乱排列的核糖体间隔序列。
