Department of Environmental Science and Engineering, Ewha Womans University, 52, Ewhayeodae-gil, Seodaemun-gu, Seoul 120-750, Republic of Korea.
Department of Environmental Science and Engineering, Ewha Womans University, 52, Ewhayeodae-gil, Seodaemun-gu, Seoul 120-750, Republic of Korea.
J Biotechnol. 2014 Mar 10;173:90-7. doi: 10.1016/j.jbiotec.2014.01.010. Epub 2014 Jan 18.
Tobermolite was characterized as a bed material for methanotrophic biofiltration. A lab-scale biofilter packed with tobermolite was operated for different operation times under identical conditions. The three different runs showed similar acclimation patterns of methane oxidation, with methane removal efficiency increasing rapidly for the first few days and peaking within three weeks, after which the efficiency remained stable. The mean methane removal capacities ranged from 766gm(-3)d(-1) to 974gm(-3)d(-1) after acclimation. Pyrosequencing indicated that the methanotrophic proportion (methanotroph/bacteria) increased to 71-94% within three weeks. Type I methanotrophs Methylocaldum and Methylosarcina were dominant during the initial growth period, then Methylocaldum alone dominated the methanotrophic community. A community comparison showed that total bacterial and methanotrophic communities were temporally stable after the initial growth period. Quantitative PCR showed that methanotrophic density increased during the first 3-4 weeks, then remained stable over 120 days. Tobermolite can provide a special habitat for the selective growth of methanotrophs, resulting in rapid acclimation. Tobermolite also allows the microbial community and methanotrophic density to remain stable, resulting in stable methane biofiltration.
将硬硅钙石作为甲烷氧化菌生物过滤的床料进行了特性描述。在相同条件下，用硬硅钙石填充的实验室规模生物过滤器在不同的运行时间下进行了操作。这三个不同的运行都表现出相似的甲烷氧化驯化模式，甲烷去除效率在最初几天迅速提高，在三周内达到峰值，之后效率保持稳定。适应期后，平均甲烷去除容量范围为 766gm(-3)d(-1)至 974gm(-3)d(-1)。焦磷酸测序表明，甲烷氧化菌的比例（甲烷氧化菌/细菌）在三周内增加到 71-94%。在初始生长阶段，I 型甲烷氧化菌甲基营养菌和甲基弧菌占优势，然后只有甲基营养菌主导甲烷氧化菌群落。群落比较表明，总细菌和甲烷氧化菌群落在初始生长阶段后具有时间稳定性。定量 PCR 显示，甲烷氧化菌密度在前 3-4 周增加，然后在 120 天内保持稳定。硬硅钙石可以为甲烷氧化菌的选择性生长提供特殊的栖息地，从而实现快速驯化。硬硅钙石还可以使微生物群落和甲烷氧化菌密度保持稳定，从而实现稳定的甲烷生物过滤。
