Sun Meng-Ting, Zhao Yu-Zhong, Yang Zhi-Man, Shi Xiao-Shuang, Wang Lin, Dai Meng, Wang Fei, Guo Rong-Bo
College of Electromechanical Engineering, Shandong Engineering Laboratory for Preparation and Application of High-Performance Carbon-Materials, Qingdao University of Science & Technology, Qingdao, China.
Shandong Industrial Engineering Laboratory of Biogas Production & Utilization, Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, China.
Front Bioeng Biotechnol. 2020 Apr 22;8:351. doi: 10.3389/fbioe.2020.00351. eCollection 2020.
Methane is a greenhouse gas and significantly contributes to global warming. Methane biofiltration with immobilized methane-oxidizing bacteria (MOB) is an efficient and eco-friendly approach for methane elimination. To achieve high methane elimination capacity (EC), it is necessary to use an exceptional support material to immobilize MOB. The MOB consortium was inoculated in biofilters to continuusly eliminate 1% () of methane. Results showed that the immobilized MOB cells outperformed than the suspended MOB cells. The biofilter packed with fly ash ceramsite (FAC) held the highest average methane EC of 4.628 g h m, which was 33.4% higher than that of the biofilter with the suspended MOB cells. The qPCR revealed that FAC surface presented the highest gene abundance, which inferred that FAC surface immobilized the most MOB biomass. The XPS and contact angle measurement indicated that the desirable surface elemental composition and stronger surface hydrophilicity of FAC might favor MOB immobilization and accordingly improve methane elimination.
甲烷是一种温室气体，对全球变暖有显著影响。利用固定化甲烷氧化菌（MOB）进行甲烷生物过滤是一种高效且环保的甲烷消除方法。为了实现高甲烷消除能力（EC），有必要使用特殊的载体材料来固定MOB。将MOB菌群接种到生物滤池中以持续消除1%（）的甲烷。结果表明，固定化MOB细胞的性能优于悬浮MOB细胞。填充粉煤灰陶粒（FAC）的生物滤池平均甲烷EC最高，为4.628 g h m，比悬浮MOB细胞生物滤池高33.4%。定量聚合酶链反应（qPCR）显示，FAC表面呈现出最高的基因丰度，这表明FAC表面固定了最多的MOB生物量。X射线光电子能谱（XPS）和接触角测量表明，FAC理想的表面元素组成和更强的表面亲水性可能有利于MOB的固定，从而提高甲烷消除效果。
