Global Top5 Research Program, Department of Environmental Science and Engineering, Ewha Womans University, 11-1 Daehyun-dong, Seodaemun-gu, Seoul, 120-750, Republic of Korea.
Appl Microbiol Biotechnol. 2014 Apr;98(7):3275-86. doi: 10.1007/s00253-013-5371-2. Epub 2013 Nov 28.
Temporal microbial succession was investigated in relation to the performance of a methane biofilter. A laboratory-scale biofilter packed with perlite was operated for 108 days, without a deliberate biomass control. The system performance was stable over the period with a mean elimination capacity of 1,563 g m(-3) day(-1), despite a temporal deterioration (45-56 days). Ribosomal-tag pyrosequencing showed that bacterial communities at days 14-28 were distinct from those of days 68-108. The accumulation of nonviable substances strongly coincided with the community change (R (2) > 0.97). Rhodobacter, Hydrogenophaga, and Methylomonas were dominated in the earlier period, while Methylocaldum and Methylococcus were abundant in the later period. The methanotrophic proportion gradually increased to 41 %, and type I methanotrophs became predominant over time. However, community structure and methanotrophic population density stably retained over time, allowing the system to keep the similar performance. Therefore, the perlite biofilter system was functionally rigid against the temporal microbial succession.
考察了与甲烷生物滤池性能相关的时间微生物演替。在没有刻意进行生物量控制的情况下，用珍珠岩填充的实验室规模生物滤池运行了 108 天。尽管存在时间上的恶化（45-56 天），但该系统在整个期间的性能都很稳定，平均去除能力为 1563 g m(-3) day(-1)。核糖体标签焦磷酸测序显示，第 14-28 天的细菌群落与第 68-108 天的细菌群落不同。不可生物降解物质的积累与群落变化强烈吻合（R (2) > 0.97）。在早期，优势菌为红杆菌、噬氢菌和甲基单胞菌，而在后期，甲基球菌和甲基杆菌丰富。甲烷氧化菌的比例逐渐增加到 41%，并且随着时间的推移，I 型甲烷氧化菌成为优势菌。然而，群落结构和甲烷氧化菌的种群密度随着时间的推移保持稳定，从而使系统保持类似的性能。因此，珍珠岩生物滤池系统在功能上对时间微生物演替具有较强的抵抗力。
