Morrison-Plummer J, Jones D H, Daly K, Tully J G, Taylor-Robinson D, Baseman J B
Department of Microbiology, University of Texas Health Science Center, San Antonio 78284.
Isr J Med Sci. 1987 May;23(5):453-7.
Species-specific proteins of Mycoplasma genitalium as well as proteins cross-reactive with M. pneumoniae have been identified using monoclonal antibodies generated against these mycoplasmas in enzyme-linked immunosorbent assays (ELISA) and Western blot analyses. Specificity of the antibodies was examined using M. hominis, M. orale M. salivarium and Acholeplasma laidlawii. A 140-kDa (kilodalton) protein of M. genitalium that appeared to be immunodominant in mice was also shown by radioimmunoprecipitation to be immunodominant in experimentally infected chimpanzees.
利用针对这些支原体产生的单克隆抗体，通过酶联免疫吸附测定（ELISA）和蛋白质印迹分析，已鉴定出生殖支原体的种特异性蛋白以及与肺炎支原体交叉反应的蛋白。使用人型支原体、口腔支原体、唾液支原体和解脲脲原体检测了抗体的特异性。生殖支原体的一种140千道尔顿（kDa）的蛋白在小鼠中似乎是免疫显性的，放射免疫沉淀法也表明该蛋白在实验感染的黑猩猩中也是免疫显性的。
