Davison P R, Cohle S D
Department of Laboratory Medicine, Blodgett Memorial Medical Center, Grand Rapids, MI.
J Forensic Sci. 1987 Sep;32(5):1426-30.
We describe a procedure for detection of fat emboli in formalin fixed tissue using osmium tetroxide postfixation. Intravascular fat in tissue postfixed in osmium tetroxide, embedded in epoxy or paraffin, and stained with toluidine blue, hematoxylin, or Oil Red O is more easily visualized than in frozen tissue that is stained with Oil Red O. With these methods, fat emboli may be detected years after the initial autopsy.
我们描述了一种使用四氧化锇后固定法检测福尔马林固定组织中脂肪栓子的方法。在经四氧化锇后固定、包埋于环氧树脂或石蜡中并用甲苯胺蓝、苏木精或油红O染色的组织中，血管内脂肪比用油红O染色的冷冻组织更容易观察到。通过这些方法，在初次尸检数年之后仍可检测到脂肪栓子。
