Abramowsky C R, Pickett J P, Goodfellow B C, Bradford W D
Hum Pathol. 1981 Aug;12(8):753-5. doi: 10.1016/s0046-8177(81)80179-7.
The presence and localization of fat in human lung tissue was evaluated by the "en bloc" staining procedure with osmium tetroxide performed with formalin fixed tissue with subsequent paraffin sectioning and with the oil red O technique performed with frozen sections. Fifty-one autopsy cases were divided into three clinical groups: group A, consisting of 17 patients with severe skeletal trauma; group B, consisting of 14 patients with minor skeletal trauma; and group C, consisting of 20 control patients without trauma. Adjacent sections of lung were selected from all cases, stained with the "en bloc" osmium tetroxide and oil red O methods, coded, and examined under the light microscope without knowledge of the clinical grouping, Stainable fat was graded on a 1 to 4+ scale, and attention was given to histologic localization in tissue sections. The "en bloc" osmium tetroxide technique revealed greater amounts of stainable lipid in clinical groups A and B and was most effective in demonstrating lipid when present in small quantities (group B). Since the method permits the employment of paraffin sections, evaluation of fine histologic detail is an advantage that is not always obtained in frozen sections. It is concluded that the "en bloc" osmium tetroxide technique is superior to the oil red O technique in terms of visualization and histologic localization of minute amounts of lipid in lung tissue.
采用四氧化锇“整体”染色法对福尔马林固定组织进行后续石蜡切片，以及采用油红O技术对冰冻切片进行处理，以此评估人体肺组织中脂肪的存在及定位情况。51例尸检病例被分为三个临床组：A组，由17例严重骨骼创伤患者组成；B组，由14例轻度骨骼创伤患者组成；C组，由20例无创伤的对照患者组成。从所有病例中选取相邻的肺组织切片，用四氧化锇“整体”染色法和油红O法进行染色，编码后在不知临床分组情况的条件下于光学显微镜下检查。可染色脂肪按1至4+级进行分级，并关注组织切片中的组织学定位。四氧化锇“整体”染色法在A组和B组临床病例中显示出更多的可染色脂质，且在脂质少量存在时（B组）显示脂质最为有效。由于该方法允许使用石蜡切片，因此能够评估精细的组织学细节，这是冰冻切片不一定具备的优势。得出的结论是，就肺组织中微量脂质的可视化和组织学定位而言，四氧化锇“整体”染色法优于油红O技术。
