Ryseff Julia K, Duncan Colleen, Sfiligoi Gabriella, Avery Paul R
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO, USA.
Vet Clin Pathol. 2014 Mar;43(1):94-100. doi: 10.1111/vcp.12114. Epub 2014 Jan 21.
A 4-year-old, female spayed Domestic Shorthair cat was presented for a 2-day history of lethargy, anorexia, and vomiting. Diagnostic assessments revealed a severe, regenerative anemia and the presence of a solitary, 3 × 4 cm splenic mass. Cytologic examination of fine-needle aspirates of the splenic mass showed sheets of pleomorphic histiocytic cells with numerous intracellular and extracellular, negatively staining to deeply basophilic, branching structures. These structures were typically of thin uniform thickness with random angled branching that formed geometrical shapes and fragments, occasionally with sharpened ends and a crystalline appearance. Septal-like divisions were irregularly spaced along some of the linear branches. The cytology was interpreted as a probable histiocytic inflammatory response to the unidentified foreign material with histiocytic sarcoma as a differential diagnosis. Histopathologic evaluation of the splenic mass resulted in a diagnosis of histiocytic sarcoma. The branching material stained positively for both iron and calcium, indicating that the material was compatible with Gamna-Gandy bodies present secondary to tumor-associated hemorrhage and the formation of calcium-iron complexes.
一只4岁已绝育的雌性家养短毛猫因出现2天的嗜睡、厌食和呕吐症状前来就诊。诊断评估显示严重的再生性贫血以及脾脏有一个3×4厘米的孤立肿块。对脾脏肿块细针穿刺抽吸物进行的细胞学检查显示，有大量多形性组织细胞片，细胞内和细胞外有许多呈阴性染色至深嗜碱性的分支结构。这些结构通常厚度均匀且薄，有随机的角状分支，形成几何形状和碎片，偶尔末端尖锐且呈晶体状。沿一些线性分支有不规则间隔的类似间隔的分隔。细胞学检查结果解释为可能是对不明外来物质的组织细胞炎性反应，鉴别诊断为组织细胞肉瘤。对脾脏肿块的组织病理学评估诊断为组织细胞肉瘤。分支物质对铁和钙均呈阳性染色，表明该物质与肿瘤相关出血继发的Gamna-Gandy小体以及钙-铁复合物的形成相符。
