Diemer P, Markoew S, Le D Q S, Qvist N
University of Southern Denmark, Odense, Denmark.
Dis Esophagus. 2015 Apr;28(3):240-5. doi: 10.1111/dote.12172. Epub 2014 Jan 22.
Repair of long-gap esophageal atresia is associated with a high degree of complications. Tissue engineering on a scaffold of a bioresorbable material could be a solution. The aim of the present study was to investigate the in vivo tissue engineering of smooth muscle cells and epithelium on a poly-ε-caprolactone mesh in rabbit esophagus. Twenty female rabbits had a window of 0.6 × 1 cm cut in the abdominal part of the esophagus. The defect was covered with a poly-ε-caprolactone mesh. The rabbits were killed on postoperative day 28-30, and mesh with surrounding esophagus was removed for histological examination. Fifteen rabbits survived the trial period. Six had no complications and had the mesh in situ. They all had ingrowth of epithelial and smooth muscle cells and an almost completely degraded mesh. Nine rabbits developed pseudo-diverticula. It proved possible to engineer both epithelial and smooth muscle cells on the poly-ε-caprolactone mesh in spite of a fast mesh degradation. The latter may be the explanation to the development of pseudo-diverticula; this is a problem that needs attention in future experimental trials.
长间隙食管闭锁的修复与高度的并发症相关。基于可生物吸收材料支架的组织工程可能是一种解决方案。本研究的目的是研究在兔食管的聚ε-己内酯网片上平滑肌细胞和上皮细胞的体内组织工程。20只雌性兔在食管腹部切一个0.6×1厘米的窗口。缺损用聚ε-己内酯网片覆盖。术后第28 - 30天处死兔子，取出带有周围食管的网片进行组织学检查。15只兔子存活至试验期结束。6只无并发症且网片原位留存。它们均有上皮细胞和平滑肌细胞向内生长且网片几乎完全降解。9只兔子出现假性憩室。尽管网片快速降解，但在聚ε-己内酯网片上培养上皮细胞和平滑肌细胞是可行的。后者可能是假性憩室形成的原因；这是未来实验研究中需要关注的一个问题。
