State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China.
Biomaterials. 2012 Nov;33(33):8363-71. doi: 10.1016/j.biomaterials.2012.08.023. Epub 2012 Aug 22.
A flexible and fibrous composite scaffold composed of poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) (PCL-PEG-PCL, PCEC) and 30 wt.% nano-hydroxyapatite (n-HA) was fabricated through electrospinning. In the present study, we investigated its in vitro and in vivo performance by means of hydrolytic degradation, muscle pouch implantation, as well as repair the calvarial defects in New Zealand white rabbits. The results demonstrated that the degradable scaffold held good biocompatibility. Qualitative analysis of bone regeneration process was performed by radiological examination and histological analysis. The results indicated that new bone formed originally from the margin of host bone, and then grew toward the center of defects. Moreover, the quantitative determination of newly formed bone was performed using statistical analysis of histological sections at predetermined time points. At 20th week, the defects of treatment group were covered with the new solid cortical bone. In comparison, the control group was filled with a large amount of cancelous bone and bone marrow. It suggested that the composite scaffold had better activity of guided bone regeneration than that of self-healing. So the electrospun PCEC/n-HA fibrous scaffold had the great potential application in bone tissue engineering.
一种由聚（ε-己内酯）-聚（乙二醇）-聚（ε-己内酯）（PCL-PEG-PCL，PCEC）和 30wt%纳米羟基磷灰石（n-HA）组成的具有弹性和纤维状的复合支架，通过静电纺丝制备而成。在本研究中，我们通过水解降解、肌肉囊植入以及修复新西兰白兔的颅顶骨缺损来研究其体外和体内性能。结果表明，可降解支架具有良好的生物相容性。通过放射学检查和组织学分析对骨再生过程进行定性分析。结果表明，新骨最初从宿主骨的边缘形成，然后向缺损的中心生长。此外，通过在预定时间点对组织学切片进行统计分析来定量测定新形成的骨。在第 20 周时，治疗组的缺损被新的坚固皮质骨覆盖。相比之下，对照组充满了大量的松质骨和骨髓。这表明与自身愈合相比，复合支架具有更好的引导骨再生活性。因此，静电纺丝 PCEC/n-HA 纤维支架在骨组织工程中有很大的潜在应用。
