Chemistry, School of Engineering and Science, Jacobs University Bremen , Campus Ring 1, 28759 Bremen, Germany.
J Agric Food Chem. 2014 Feb 12;62(6):1261-71. doi: 10.1021/jf4053989. Epub 2014 Jan 31.
Bottle gourd, Lagenaria siceraria Stand. (Cucurbitaceae), fruit is used in folk medicines and for culinary purposes in Asia. The phenolics of bottle gourd fruit were investigated qualitatively by LC-MS(n). Twenty-two phenolic glycosides were detected and characterized on the basis of their unique fragmentation pattern in the negative ion mode tandem MS spectra. Twenty of them were extracted for the first time from this source, and twelve of them have not been reported previously in nature. It was also possible to distinguish between the individual classes of isobaric phenolic glycosides by tandem and high-resolution mass spectrometry. In this study we also discuss the mass spectrometric fragmentation mechanism of 6-(hydroxycinnamoyl)glucoses. This is the first report of the full characterization of phenolic glycosides of bottle gourd fruit by LC-MS²⁻⁴.
葫芦，Lagenaria siceraria Stand.（葫芦科），果实被用于亚洲的民间药物和烹饪用途。通过 LC-MS(n)对葫芦果实的酚类物质进行了定性研究。根据负离子模式串联 MS 光谱中的独特碎片模式，检测并表征了 22 种酚糖苷。其中 20 种为首次从该来源中提取，其中 12 种以前在自然界中尚未报道过。通过串联和高分辨率质谱也可以区分个别类别的等质异位酚糖苷。在本研究中，我们还讨论了 6-(羟基肉桂酰基)葡萄糖的质谱裂解机制。这是首次通过 LC-MS²⁻⁴对葫芦果实酚糖苷进行全面表征的报道。
