Caon Thiago, Kaiser Samuel, Feltrin Clarissa, de Carvalho Annelise, Sincero Thaís Cristine Marques, Ortega George González, Simões Cláudia Maria Oliveira
Laboratório de Virologia Aplicada, Universidade Federal de Santa Catarina, Campus Universitário, Trindade, 88040-900 Florianópolis-SC, Brazil.
Laboratório de Desenvolvimento Galênico, Departamento de Controle e Produção de Medicamentos, Universidade Federal do Rio Grande do Sul (UFRGS), Faculdade de Farmácia, Santana, 90610-000 Porto Alegre-RS, Brazil.
Food Chem Toxicol. 2014 Apr;66:30-5. doi: 10.1016/j.fct.2014.01.013. Epub 2014 Jan 18.
Uncaria tomentosa have been used to treat viral diseases such as herpes due to multiple pharmacological effects, but its therapeutic efficacy against this virus have not been reported yet. Thus, in vitro antiherpetic activity of hydroethanolic extract from barks, purified fractions of quinovic acid glycosides and oxindole alkaloids was evaluated by plaque reduction assay, including mechanistic studies (virucidal, attachment and penetration action). Once exposure to physical agents might lead to reactivation of the herpetic infection, antimutagenic effect (pre-, simultaneous and post-treatment protocols) was also evaluated by Comet assay. The antiherpetic activity from the samples under investigation seemed to be associated with the presence of polyphenols or their synergistic effect with oxindole alkaloids or quinovic acid glycosides, once both purified fractions did not present activity when evaluated alone. Inhibition of viral attachment in the host cells was the main mechanism of antiviral activity. Although both purified fractions displayed the lowest antimutagenic activity in pre and simultaneous treatment, they provided a similar effect to that of cat's claw hydroethanolic extract in post-treatment. Given that purified fractions may result in a reduced antiherpetic activity, the use of cat's claw hydroethanolic extract from barks should be prioritized in order to obtain a synergistic effect.
由于具有多种药理作用，绒毛钩藤已被用于治疗疱疹等病毒性疾病，但其对该病毒的治疗效果尚未见报道。因此，通过蚀斑减少试验评估了树皮乙醇提取物、奎诺酸糖苷和氧化吲哚生物碱纯化组分的体外抗疱疹活性，包括作用机制研究（杀病毒、附着和穿透作用）。由于接触物理因素可能导致疱疹感染重新激活，还通过彗星试验评估了抗诱变作用（预处理、同时处理和后处理方案）。所研究样品的抗疱疹活性似乎与多酚的存在或其与氧化吲哚生物碱或奎诺酸糖苷的协同作用有关，因为两个纯化组分单独评估时均无活性。抑制病毒在宿主细胞中的附着是抗病毒活性的主要机制。虽然两个纯化组分在预处理和同时处理中显示出最低的抗诱变活性，但它们在后处理中提供了与猫爪乙醇提取物类似的效果。鉴于纯化组分可能导致抗疱疹活性降低，为了获得协同效应，应优先使用树皮的猫爪乙醇提取物。
