Kour Jasbir, Ali Md Niamat, Ganaie Hilal Ahmad, Tabassum Nahida
Cytogenetics & Molecular Biology Research Laboratory, Centre of Research for Development, University of Kashmir, Srinagar 190 006, JK, India.
Department of Pharmaceutical Sciences, University of Kashmir, Srinagar 190 006, JK, India.
Toxicol Rep. 2017 May 11;4:226-233. doi: 10.1016/j.toxrep.2017.05.001. eCollection 2017.
In the present study, we evaluated the potential of the plant against the cytotoxic and mutagenic effects induced by cyclophosphamide (chemotherapeutic agent) in the bone marrow cells of mice using the Chromosome assay (CA) and Mitotic index (MI) as the biomarkers. The study was performed following 3 protocols: pre-treatment, simultaneous treatment and post-treatment with the ethanolic extract of the plant. The results demonstrated that the plant extract was not cytotoxic and mutagenic and has a protective effect against the mutagenicity induced by cyclophosphamide in pre, simultaneous and post treatments and against its cytotoxicity as well. Because of its ability to prevent chromosomal damage is likely to open an interesting field concerning its possible use in clinical applications, most importantly in cancer as a chemopreventive agent or even as a coadjuvant to chemotherapy to reduce the side effects associated with it.
在本研究中，我们以染色体分析（CA）和有丝分裂指数（MI）作为生物标志物，评估了该植物对环磷酰胺（化疗药物）诱导的小鼠骨髓细胞细胞毒性和致突变性的潜在影响。该研究按照3种方案进行：用该植物的乙醇提取物进行预处理、同时处理和后处理。结果表明，该植物提取物无细胞毒性和致突变性，并且在预处理、同时处理和后处理中对环磷酰胺诱导的致突变性以及对其细胞毒性均具有保护作用。由于其预防染色体损伤的能力，可能会开辟一个有趣的领域，涉及其在临床应用中的可能用途，最重要的是在癌症中作为化学预防剂，甚至作为化疗的辅助剂以减少与之相关的副作用。
