基质辅助激光解吸电离成像质谱：实现新时代发现的空间分子分析技术
MALDI imaging mass spectrometry: spatial molecular analysis to enable a new age of discovery.
作者信息
Gessel Megan M, Norris Jeremy L, Caprioli Richard M
机构信息
National Research Resource for Imaging Mass Spectrometry, Mass Spectrometry Research Center, Vanderbilt University School of Medicine, 9160 Medical Research Building III, 465 21st Avenue South, Nashville, TN 37232-8575, United States; Department of Biochemistry, Vanderbilt University School of Medicine, 9160 Medical Research Building III, 465 21st Avenue South, Nashville, TN 37232-8575, United States.
National Research Resource for Imaging Mass Spectrometry, Mass Spectrometry Research Center, Vanderbilt University School of Medicine, 9160 Medical Research Building III, 465 21st Avenue South, Nashville, TN 37232-8575, United States; Department of Biochemistry, Vanderbilt University School of Medicine, 9160 Medical Research Building III, 465 21st Avenue South, Nashville, TN 37232-8575, United States.
出版信息
J Proteomics. 2014 Jul 31;107:71-82. doi: 10.1016/j.jprot.2014.03.021. Epub 2014 Mar 29.
Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) combines the sensitivity and selectivity of mass spectrometry with spatial analysis to provide a new dimension for histological analyses to provide unbiased visualization of the arrangement of biomolecules in tissue. As such, MALDI IMS has the capability to become a powerful new molecular technology for the biological and clinical sciences. In this review, we briefly describe several applications of MALDI IMS covering a range of molecular weights, from drugs to proteins. Current limitations and challenges are discussed along with recent developments to address these issues. This article is part of a Special Issue entitled: 20years of Proteomics in memory of Viatliano Pallini. Guest Editors: Luca Bini, Juan J. Calvete, Natacha Turck, Denis Hochstrasser and Jean-Charles Sanchez.
基质辅助激光解吸/电离成像质谱（MALDI IMS）将质谱的灵敏度和选择性与空间分析相结合，为组织学分析提供了一个新的维度，能够无偏倚地可视化组织中生物分子的排列。因此，MALDI IMS有潜力成为生物和临床科学领域一项强大的新型分子技术。在本综述中，我们简要描述了MALDI IMS的几种应用，涵盖了从药物到蛋白质的一系列分子量范围。同时讨论了当前的局限性和挑战以及为解决这些问题的最新进展。本文是名为“纪念维亚特利亚诺·帕利尼的蛋白质组学20年”特刊的一部分。客座编辑：卢卡·比尼、胡安·J·卡尔韦特、娜塔莎·图尔克、丹尼斯·霍斯特拉斯和让-查尔斯·桑切斯。
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