From the Departments of *Breast Surgery, †Hepatobilliary Surgery, and ‡Oncology Surgery, the First Affiliated Hospital of China Medical University, Shenyang, China.
J Investig Med. 2014 Mar;62(3):627-31. doi: 10.2310/JIM.0000000000000046.
Interleukin 27 (IL-27), belonging to the IL-12 family, exerts a critical role in immune regulation of infection, autoimmunity, and tumor. In this study, we aimed to investigate the roles of IL-27 and vascular endothelial growth factor (VEGF) in breast cancer.
The serum levels of IL-27 and VEGF in 150 patients with breast cancer and 90 control subjects were measured by an enzyme-linked immunosorbent assay. Estrogen receptor, progesterone receptor, and human epithelial growth factor receptor 2 were measured by immunohistochemistry. Clinical stage, tumor size, lymph node metastasis, grade, and histological type were also recorded.
The serum levels of IL-27 and VEGF were significantly greater in breast cancer patients than in the control group. In addition, there was a significant correlation between IL-27 and VEGF, and serum IL-27 and VEGF levels were associated with the clinical stage of breast cancer. Moreover, the serum levels of IL-27 were especially elevated in breast cancer patients who were estrogen receptor-positive and progesterone receptor-positive. Furthermore, the serum levels of IL-27 and VEGF decreased after patients with breast cancer had modified radical mastectomy.
Interleukin 27 may be a new prognostic biomarker of breast cancer and a promising target to limit both angiogenesis and tumor growth.
白细胞介素 27（IL-27）属于 IL-12 家族，在感染、自身免疫和肿瘤的免疫调节中发挥着关键作用。在本研究中，我们旨在研究 IL-27 和血管内皮生长因子（VEGF）在乳腺癌中的作用。
采用酶联免疫吸附试验检测 150 例乳腺癌患者和 90 例对照者血清中 IL-27 和 VEGF 的水平。采用免疫组织化学法检测雌激素受体、孕激素受体和人表皮生长因子受体 2。还记录了临床分期、肿瘤大小、淋巴结转移、分级和组织学类型。
乳腺癌患者血清中 IL-27 和 VEGF 的水平明显高于对照组。此外，IL-27 和 VEGF 之间存在显著相关性，且血清 IL-27 和 VEGF 水平与乳腺癌的临床分期有关。此外，在雌激素受体阳性和孕激素受体阳性的乳腺癌患者中，血清 IL-27 水平特别升高。而且，乳腺癌患者行改良根治性乳房切除术治疗后，血清中 IL-27 和 VEGF 的水平下降。
白细胞介素 27 可能是乳腺癌的一个新的预后生物标志物，也是限制血管生成和肿瘤生长的有前途的靶点。
