Endo Masayuki, Feola Andrew, Sindhwani Nikhil, Manodoro Stefano, Vlacil Jarek, Engels Alexander Carl, Claus Filip, Deprest Jan A
Pelvic Floor Unit, A Programme of Gynaecology, Urology and Gastro-Enterology, University Hospitals Leuven, 3000, Leuven, Belgium.
Int Urogynecol J. 2014 Jun;25(6):737-43. doi: 10.1007/s00192-013-2293-6. Epub 2014 Jan 22.
Our aim was to analyze the apparent contraction of meshes in vivo after abdominal wall reconstruction and evaluate histological and biomechanical properties after explantation.
Nine New Zealand female rabbits underwent repair of two full-thickness 25 × 30-mm midline defects in the upper and lower parts of the abdomen. These were primarily overlaid by 35 × 40-mm implants of a polyvinylidene fluoride (PVDF) DynaMesh (n = 6) or polypropylene meshes Ultrapro (n = 6) and Marlex (n = 6). Edges of the meshes were secured with iron(II,III) oxide (Fe(3)O(4))-loaded PVDF sutures. Magnetic resonance images (MRIs) were taken at days 2, 30 and 90 after implantation. The perimeter of the mesh was traced using a 3D spline curve. The apparent surface area or the area within the PVDF sutures was compared with the initial size using the one-sample t test. A two-way repeat analysis of variance (ANOVA) was used to compare the apparent surface area over time and between groups.
PVDF meshes and sutures with Fe(3)O(4) could be well visualized on MRI. DynaMesh and Marlex each had a 17 % decrease in apparent surface area by day 2 (p < 0.001 and p = 0.001), respectively, which persisted after day 90. Whereas there was a decrease in apparent surface area in Ultrapro, it did not reach significance until day 90 (p = 0.01). Overall, the apparent surface area decreased 21 % in all meshes by day 90. No differences in histological or biomechanical properties were observed at day 90.
There was a reduction in the apparent surface area between implantation and day 2, indicating that most mesh deformation occurs prior to tissue in-growth.
我们的目的是分析腹壁重建后体内网片的明显收缩情况，并评估取出后的组织学和生物力学特性。
9只新西兰雌性兔子接受了腹部上下部两个25×30毫米全层中线缺损的修复。这些缺损主要用35×40毫米的聚偏二氟乙烯（PVDF）DynaMesh植入物（n = 6）、聚丙烯网片Ultrapro（n = 6）和Marlex（n = 6）覆盖。网片边缘用负载四氧化三铁（Fe(3)O(4)）的PVDF缝线固定。在植入后第2天、30天和90天进行磁共振成像（MRI）检查。使用三维样条曲线描绘网片的周长。使用单样本t检验将网片的表观表面积或PVDF缝线内的面积与初始尺寸进行比较。使用双向重复方差分析（ANOVA）比较不同时间和不同组之间的表观表面积。
含Fe(3)O(4)的PVDF网片和缝线在MRI上能够很好地显示。到第2天，DynaMesh和Marlex的表观表面积分别减少了17%（p < 0.001和p = 0.001），在第90天后仍持续减少。Ultrapro的表观表面积也有减少，但直到第90天才达到显著水平（p = 0.01）。总体而言，到第90天所有网片的表观表面积减少了21%。在第90天未观察到组织学或生物力学特性的差异。
从植入到第2天，表观表面积减少，表明大多数网片变形发生在组织长入之前。
