Siddiqui A A, Karcz S R, Podesta R B
Department of Zoology, University of Western Ontario, London, Canada.
Mol Biochem Parasitol. 1987 Aug;25(1):19-28. doi: 10.1016/0166-6851(87)90014-4.
Gene expression and its regulation was studied in the rat tapeworm, Hymenolepis diminuta, during strobilization. RNAs extracted from the different developmental stages of the parasite were translated in vitro in a rabbit reticulocyte lysate system. Two-dimensional patterns of the translational products were compared with the 2-dimensional patterns obtained by metabolic labelling with [35S]methionine. The results indicated a post-transcriptional regulation of gene expression during strobilization in this parasite. Gene expression and its regulation was also studied in H. diminuta obtained from 'non-permissive' hosts (mice) and immune suppressed mice and compared with the parasites of the same age obtained from 'permissive' rats hosts. The 2-dimensional patterns of the in vitro translation products, obtained by translating the RNA of different groups of parasites, were compared with the patterns of gene products obtained from parasites using [35S]methionine metabolic labelling. The results indicated a massive post-transcriptional regulation of gene expression, the latter inhibited in parasites obtained from normal mice, but not in immune suppressed mice.
在链状带绦虫发育过程中，对微小膜壳绦虫的基因表达及其调控进行了研究。从该寄生虫不同发育阶段提取的RNA，在兔网织红细胞裂解物系统中进行体外翻译。将翻译产物的二维图谱与用[35S]甲硫氨酸进行代谢标记所获得的二维图谱进行比较。结果表明，在该寄生虫发育过程中存在转录后基因表达调控。还对从“非允许性”宿主（小鼠）和免疫抑制小鼠获得的微小膜壳绦虫进行了基因表达及其调控的研究，并与从“允许性”大鼠宿主获得的同龄寄生虫进行比较。通过翻译不同组寄生虫的RNA所获得的体外翻译产物的二维图谱，与用[35S]甲硫氨酸代谢标记从寄生虫获得的基因产物图谱进行比较。结果表明存在大量的转录后基因表达调控，在从正常小鼠获得的寄生虫中，基因表达受到抑制，但在免疫抑制小鼠中则不受抑制。
