Atkinson B G, Podesta R B
Mol Biochem Parasitol. 1982 Jul;6(1):33-43. doi: 10.1016/0166-6851(82)90051-2.
This study is the first demonstration of individual gene products synthesized by Hymenolepis diminuta. To identify proteins synthesized specifically by H. diminuta, we radiolabeled adult worms with [14C]leucine in vitro. Subsequent one- and two-dimensional gel electrophoretic separation and staining of the protein constituents from lysates of whole worms, brush border denuded worms, and brush border fractions extracted from whole worms demonstrates the diversity of the proteins in each of these lysates. Fluorographic analysis of the radioactivity in the same polyacrylamide gels identified the protein constituents synthesized by H. diminuta. The results from this study disclose and characterize, by molecular weight and isoelectric point, 17 polypeptides which are synthesized by H. diminuta and associated with its surface brush border.
本研究首次证明了微小膜壳绦虫合成的个体基因产物。为了鉴定由微小膜壳绦虫特异性合成的蛋白质，我们在体外用[14C]亮氨酸对成虫进行放射性标记。随后对完整虫体裂解物、刷状缘去除的虫体以及从完整虫体中提取的刷状缘部分的蛋白质成分进行一维和二维凝胶电泳分离及染色，证明了这些裂解物中蛋白质的多样性。对同一聚丙烯酰胺凝胶中的放射性进行荧光自显影分析，确定了微小膜壳绦虫合成的蛋白质成分。本研究结果通过分子量和等电点揭示并表征了由微小膜壳绦虫合成并与其表面刷状缘相关的17种多肽。
