Javor Juraj, Králinský Karol, Sádová Eva, Červeňová Oľga, Bucová Mária, Olejárová Michaela, Buc Milan, Liptáková Adriana
Department of Immunology, Faculty of Medicine, Comenius University in Bratislava, Odborárske nám. 14, 81108, Bratislava, Slovakia,
Folia Microbiol (Praha). 2014 Jul;59(4):307-13. doi: 10.1007/s12223-014-0303-9. Epub 2014 Jan 22.
Interleukin-10 (IL-10) is a potent inhibitor of leukocyte chemotaxis, bacterial killing in phagocytes and synthesis of pro-inflammatory cytokines and chemokines, and recent studies have suggested an important role for this immunoregulatory cytokine in the pathogenesis of urinary tract infections (UTIs). Therefore, the gene encoding IL-10 (IL10) is an attractive candidate for association studies attempting to identify susceptibility genes conferring risk of UTIs. In this case-control study, we aimed to investigate the association of single nucleotide polymorphisms (SNPs) in the promoter region of IL10 with acute pyelonephritis in the Slovak population. Polymerase chain reaction with sequence-specific primers was used to analyse IL10 -1082A/G (rs1800896), -819C/T (rs1800871) and -592C/A (rs1800872) SNPs in 147 children with acute pyelonephritis and 215 healthy controls. Comparison of patients with healthy controls using the logistic regression analysis revealed significantly increased risk of developing recurrent attacks of acute pyelonephritis for -1082 G allele in a dominant genetic model GG (GG + AG vs. AA, P = 0.019, odds ratio (OR) = 2.26). A similar tendency was also found when the recurrent acute pyelonephritis subgroup was compared to episodic pyelonephritis cases (GG + AG vs. AA, P = 0.009, OR = 3.38). In conclusion, our results suggest that IL10 -1082 A/G SNP is a susceptibility factor for development of recurrent attacks of acute pyelonephritis.
白细胞介素-10（IL-10）是白细胞趋化、吞噬细胞杀菌以及促炎细胞因子和趋化因子合成的强效抑制剂，近期研究表明这种免疫调节细胞因子在尿路感染（UTIs）的发病机制中起重要作用。因此，编码IL-10的基因（IL10）是关联研究中极具吸引力的候选基因，这类研究旨在识别赋予UTIs风险的易感基因。在这项病例对照研究中，我们旨在调查斯洛伐克人群中IL10启动子区域单核苷酸多态性（SNP）与急性肾盂肾炎的关联。采用序列特异性引物聚合酶链反应分析了147例急性肾盂肾炎患儿和215例健康对照者的IL10 -1082A/G（rs1800896）、-819C/T（rs1800871）和-592C/A（rs1800872）SNP。通过逻辑回归分析比较患者与健康对照者发现，在显性遗传模型GG中，-1082 G等位基因使急性肾盂肾炎复发发作的风险显著增加（GG + AG vs. AA，P = 0.019，比值比（OR）= 2.26）。将复发性急性肾盂肾炎亚组与偶发性肾盂肾炎病例进行比较时也发现了类似趋势（GG + AG vs. AA，P = 0.009, OR = 3.38）。总之，我们的结果表明IL1家族的IL10 -1082 A/G SNP是急性肾盂肾炎复发发作的易感因素。
