Wang Hu-jun, Gao Wei-cheng, Ma Shao-lin
Department of Plastic Surgery of the First Teaching Hospital, Xinjiang Medical University, Urumqi, 830054, China.
Chin J Integr Med. 2015 Jul;21(7):537-41. doi: 10.1007/s11655-014-1622-4. Epub 2014 Jan 21.
To investigate whether administrating Abnormal Savda Munziq (ASMq), a traditional Uighur herbal preparation used for the prevention or treatment of diseases, affects hypertrophic scar (HTS) formation by using an established rabbit ear model.
The HTS rabbit model was created by circular fullthickness skin excisions on both ears of rabbits. Twenty rabbits were randomized into four groups, with 5 rabbits and 60 wounds in each group. Group A was the control group, treated with normal saline daily. Groups B, C, and D were the treatment groups at three different doses of ASMq (400, 800, and 1200 mg/kg body weight, respectively, daily, by gastrogavage). Twenty wounds were randomly chosen from each group on the 40th day after treatment and specimen were examined. Scar elevation index (SEI) was analyzed with histological assessment, and ultrastructure analysis was analyzed with a transmission electron microscopy.
Groups B, C, and D demonstrated significant reductions in SEI as compared with the control group at 35.9% (P=0.0212), 48.2% (P=0.0108), and 52.7% (P=0.0103), respectively in a dose-response manner. SEI was lowered in Group D compared with Group B with a significant difference (P=0.015). However, there were no significant differences between Groups B and C, or between Groups C and D. Histological analysis showed that highdose ASMq (1200 mg/kg) could enhance the softening of HTS of rabbit ears and increase the compliance as shown in general. Ultrastructure analysis showed that with increased ASMq dose, the fibroblasts, pro-collagen, collagen, endoplasmic reticulum and ribosomes were reduced gradually.
Orally administered ASMq significantly reduces the severity of HTS in the rabbit ear model. The findings of this study may have clinical implications on the management of human HTS.
通过建立兔耳模型，研究用于预防或治疗疾病的传统维吾尔草药制剂异常黑胆质成熟剂（ASMq）是否影响增生性瘢痕（HTS）的形成。
通过在兔双耳进行圆形全层皮肤切除建立HTS兔模型。20只兔随机分为4组，每组5只兔、60个伤口。A组为对照组，每日用生理盐水处理。B、C、D组为治疗组，分别每日经胃管给予三种不同剂量的ASMq（分别为400、800和1200mg/kg体重）。治疗后第40天从每组随机选取20个伤口并检查标本。通过组织学评估分析瘢痕隆起指数（SEI），并通过透射电子显微镜进行超微结构分析。
与对照组相比，B、C、D组的SEI分别以剂量反应方式显著降低，降低幅度分别为35.9%（P = 0.0212）、48.2%（P = 0.0108）和52.7%（P = 0.0103）。D组的SEI低于B组，差异有统计学意义（P = 0.015）。然而，B组和C组之间、C组和D组之间无显著差异。组织学分析表明，高剂量ASMq（1200mg/kg）总体上可增强兔耳HTS的软化并增加顺应性。超微结构分析表明，随着ASMq剂量增加，成纤维细胞、前胶原、胶原、内质网和核糖体逐渐减少。
口服ASMq可显著降低兔耳模型中HTS的严重程度。本研究结果可能对人类HTS的治疗具有临床意义。
