无细胞脂肪提取物和富血小板纤维蛋白对实验性兔耳伤口模型瘢痕成熟的影响。
Effects of Cell-Free Fat Extract and Platelet-Rich Fibrin on Scar Maturation in an Experimental Rabbit Ear Wound Model.
作者信息
Wei Zhenni, Zhang Mingliang, Chen Minjian, Song Yong, Wang Yi
机构信息
Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, People's Republic of China.
Fujian Obstetrics and Gynecology Hospital, Fuzhou, Fujian, People's Republic of China.
出版信息
Clin Cosmet Investig Dermatol. 2024 Dec 17;17:2901-2909. doi: 10.2147/CCID.S489625. eCollection 2024.
BACKGROUND
Multiple methods have been used to treat hypertrophic scarring; however, an optimal treatment method remains to be established. We aimed to research and compare the effects of cell-free fat extract (CEFFE) and platelet-rich fibrin (PRF) on hypertrophic scar formation based on histomorphological analysis in this study.
METHODS
Twelve rabbits were divided into four groups randomly. (CEFFE+PRF group, n=3; CEFFE group, n=3, PRF group, n=3 and Control group, n=3). After the ear hypertrophic scar model were established, the two ears of each rabbit in the four groups were injected with CEFFE 0.05 mL/cm + PRF 0.05 mL/cm, CEFFE 0.1 mL/cm, PRF 0.1 mL/cm, and saline 0.1 mL/cm, respectively. The scar elevation index and histological analysis using hematoxylin-eosin and Masson staining were evaluated after injection on day 40.
RESULTS
The CEFFE+PRF group was significantly more effective in the prevention of pathological scar formation than the CEFFE-only, PRF-only, and control groups in terms of capillary count, collagen organization, fibroblast count, and scar elevation index (<0.05).
DISCUSSION
CEFFE combined with PRF was the most effective treatment for the prevention of hypertrophic scar formation in our study.
背景
已经采用多种方法治疗肥厚性瘢痕；然而，最佳治疗方法仍有待确定。在本研究中，我们旨在基于组织形态学分析，研究并比较无细胞脂肪提取物（CEFFE）和富血小板纤维蛋白（PRF）对肥厚性瘢痕形成的影响。
方法
将12只兔子随机分为四组。（CEFFE+PRF组，n=3；CEFFE组，n=3，PRF组，n=3，对照组，n=3）。建立耳部肥厚性瘢痕模型后，四组中每只兔子的两只耳朵分别注射0.05 mL/cm的CEFFE + 0.05 mL/cm的PRF、0.1 mL/cm的CEFFE、0.1 mL/cm的PRF和0.1 mL/cm的生理盐水。在注射后第40天评估瘢痕隆起指数，并使用苏木精-伊红染色和Masson染色进行组织学分析。
结果
在毛细血管计数、胶原组织、成纤维细胞计数和瘢痕隆起指数方面，CEFFE+PRF组在预防病理性瘢痕形成方面比仅使用CEFFE组、仅使用PRF组和对照组显著更有效（<0.05）。
讨论
在我们的研究中，CEFFE联合PRF是预防肥厚性瘢痕形成最有效的治疗方法。
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