Department of Analytical Chemistry, Chemical Faculty, Gdańsk University of Technology, 80952, Gdańsk, Poland,
Appl Biochem Biotechnol. 2014 Mar;172(6):2849-65. doi: 10.1007/s12010-013-0712-2. Epub 2014 Jan 22.
The aim of this study was to investigate the possibility to use the bioactive components from cape gooseberry (Physalis peruviana), blueberry (Vaccinium corymbosum), and cranberry (Vaccinium macrocarpon) extracts as a novel source against oxidation in food supplementation. The quantitative analysis of bioactive compounds (polyphenols, flavonoids, flavanols, carotenoids, and chlorophyll) was based on radical scavenging spectrophometric assays and mass spectrometry. The total phenolic content was the highest (P < 0.05) in water extract of blueberries (46.6 ± 4.2 mg GAE/g DW). The highest antioxidant activities by 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay and Cupric reducing antioxidant capacity were in water extracts of blueberries, showing 108.1 ± 7.2 and 131.1 ± 9.6 μMTE/g DW with correlation coefficients of 0.9918 and 0.9925, and by β-carotene linoleate assay at 80.1 ± 6.6 % with correlation coefficient of 0.9909, respectively. The water extracts of berries exhibited high binding properties with human serum albumin in comparison with quercetin. In conclusion, the bioactive compounds from a relatively new source of gooseberries in comparison with blueberries and cranberries have the potential as food supplementation for human health. The antioxidant and binding activities of berries depend on their bioactive compounds.
本研究旨在探讨利用仙人掌果（Physalis peruviana）、蓝莓（Vaccinium corymbosum）和蔓越莓（Vaccinium macrocarpon）提取物中的生物活性成分作为食品补充剂中抗氧化的新来源的可能性。生物活性化合物（多酚、类黄酮、黄烷醇、类胡萝卜素和叶绿素）的定量分析基于自由基清除光谱测定法和质谱法。蓝莓水提取物中的总酚含量最高（P < 0.05），为 46.6 ± 4.2 mg GAE/g DW。通过 2,2-二苯基-1-苦基肼自由基清除测定法和铜还原抗氧化能力测定法，蓝莓水提取物的抗氧化活性最高，分别为 108.1 ± 7.2 和 131.1 ± 9.6 μMTE/g DW，相关系数分别为 0.9918 和 0.9925，β-胡萝卜素亚油酸法测定的抑制率为 80.1 ± 6.6 %，相关系数为 0.9909。与槲皮素相比，浆果的水提取物与人血清白蛋白具有更高的结合性能。总之，与蓝莓和蔓越莓相比，仙人掌果中的生物活性化合物具有作为人类健康食品补充剂的潜力。浆果的抗氧化和结合活性取决于其生物活性化合物。
