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采用高效液相色谱-二极管阵列检测-质谱联用(HPLC-DAD-MS)、高效液相色谱-ABTS 或 FC 柱后衍生化和 TLC-DPPH 可视化法研究波兰蓝果忍冬基因型蜂蜜的酚类成分和抗氧化性能。

Phenolic composition and antioxidant properties of Polish blue-berried honeysuckle genotypes by HPLC-DAD-MS, HPLC postcolumn derivatization with ABTS or FC, and TLC with DPPH visualization.

机构信息

Department of Food Chemistry, Technology and Biotechnology, Chemical Faculty, Gdansk University of Technology, Gdansk, Poland.

出版信息

J Agric Food Chem. 2012 Feb 22;60(7):1755-63. doi: 10.1021/jf2039839. Epub 2012 Feb 8.

DOI:10.1021/jf2039839
PMID:22264130
Abstract

In this study, different Polish cultivars of blue-berried honeysuckles (Lonicera caerulea L.), wild and bog bilberry, were analyzed for bioactive compounds. The chemical properties verified included composition of anthocyanins and other polyphenols, antioxidant activity, and profiles of antioxidants by HPLC postcolumn derivatization or TLC. The antioxidant activities of different blue-berried honeysuckle cultivars were similar to that of wild-growing bilberries (ranging from 170 to 417 μmol TE/g dm in ABTS and from 93 to 166 μmol TE/g dm in DPPH and Folin-Ciocalteu tests). The major anthocyanin in the blue-berried honeysuckle was cyanidin-3-glucoside, which constituted 84-92% of the total anthocyanins. The TLC and HPLC postcolumn antioxidant profiles indicated that anthocyanins are the major antioxidants in all berries studied. Wild berries and the cultivars of the blue-berried honeysuckles are also a similar source of such minerals as K, Mg, and Ca.

摘要

在这项研究中,对不同波兰品种的蓝果忍冬(Lonicera caerulea L.)、野生和沼泽越橘进行了生物活性化合物分析。验证的化学性质包括花色苷和其他多酚的组成、抗氧化活性以及通过 HPLC 柱后衍生化或 TLC 分析的抗氧化剂谱。不同蓝果忍冬品种的抗氧化活性与野生越橘相似(ABTS 中的范围为 170 至 417 μmol TE/g dm,DPPH 和 Folin-Ciocalteu 测试中的范围为 93 至 166 μmol TE/g dm)。蓝果忍冬中的主要花色苷是矢车菊素-3-葡萄糖苷,占总花色苷的 84-92%。TLC 和 HPLC 柱后抗氧化剂谱表明,花色苷是所有研究浆果中的主要抗氧化剂。野生浆果和蓝果忍冬品种也是 K、Mg 和 Ca 等矿物质的相似来源。

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