使用苏木精和伊红染色以及抗磷酸化组蛋白3免疫组化染色，对薄转移性黑色素瘤与无转移的薄黑色素瘤的肿瘤有丝分裂率进行分析。
Analysis of tumor mitotic rate in thin metastatic melanomas compared with thin melanomas without metastasis using both the hematoxylin and eosin and anti-phosphohistone 3 IHC stain.
作者信息
Thareja Sumeet, Zager Jonathan S, Sadhwani Divya, Thareja Shalini, Chen Ren, Marzban Suroosh, Jukic Drazen M, Glass Lewis F, Messina Jane
机构信息
*Department of Dermatology, University of South Florida, Tampa, FL; †Department of Cutaneous Oncology, Sarcoma Program, Moffitt Cancer Center; ‡University of Central Florida College of Medicine, Tampa, FL; §Emory University College of Medicine; ¶Biostatistics Core, Clinical and Translational Science Institute, University of South Florida College of Medicine, Tampa, FL; ‖University of South Florida College of Medicine, Tampa, FL; **Department of Pathology and Cell Biology, University of South Florida, Tampa, FL; ††Department of Dermatology, James A. Haley VAMC; ‡‡Department of Dermatology, Moffitt Cancer Center; §§Department of Dermatology, University of South Florida, Tampa, FL; and ¶¶Department of Pathology, Cell Biology and Dermatology, University of South Florida Morsani College of Medicine, Tampa, FL.
出版信息
Am J Dermatopathol. 2014 Jan;36(1):64-7. doi: 10.1097/DAD.0b013e31829433b6.
Studies have suggested that elevated tumor mitotic rate (MR) is linked to overall survival in thin melanoma. Recently, promising data regarding anti-phosphohistone 3 (pHH3) immunohistochemistry and its ability to aid in calculation of MR have emerged. The authors retrospectively analyzed original biopsies from 13 thin melanomas with positive sentinel node (SN) status and 16 thin melanomas with negative SN status. Both anti-pHH3 immunohistochemistry and the hematoxylin and eosin (H&E) stain were used to evaluate MR by 2 dermatopathologists blinded to SN status using the "hot spot" method. Intraclass coefficient values were attained to measure interobserver concordance and reliability of the pHH3 stain. By generating a receiver operating characteristic curve and analyzing the overall area under the curve, pHH3 was found to have good interobserver reliability. The relationship between MR and SN involvement was also evaluated, but this correlation was not statistically significant.
研究表明，肿瘤有丝分裂率（MR）升高与薄型黑色素瘤的总生存期相关。最近，关于抗磷酸化组蛋白H3（pHH3）免疫组化及其辅助计算MR能力的有前景的数据已出现。作者回顾性分析了13例前哨淋巴结（SN）阳性的薄型黑色素瘤和16例SN阴性的薄型黑色素瘤的原始活检标本。两名对SN状态不知情的皮肤病理学家使用“热点”方法，通过抗pHH3免疫组化和苏木精-伊红（H&E）染色来评估MR。获得组内相关系数值以测量观察者间的一致性和pHH3染色的可靠性。通过生成受试者工作特征曲线并分析曲线下总面积，发现pHH3具有良好的观察者间可靠性。还评估了MR与SN受累之间的关系，但这种相关性无统计学意义。
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