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研究伊朗萨里市自来水真菌菌群,以了解其是否为医院真菌的潜在储源。

Study on fungal flora of tap water as a potential reservoir of fungi in hospitals in Sari city, Iran.

机构信息

Department of Medical Mycology and Parasitology, School of Medicine, Mazandaran University of Medical Sciences, Km 18 Khazarabad Road, P.O. Box: 48175-1665, Sari, Iran.

Department of Environmental Health, School of Health, Mazandaran University of Medical Sciences, Sari, Iran.

出版信息

J Mycol Med. 2011 Mar;21(1):10-4. doi: 10.1016/j.mycmed.2010.12.001. Epub 2011 Jan 22.

Abstract

OBJECTIVE

The aim of the present investigation was to evaluate the fungal flora of tap water from university hospitals of Sari city, Iran.

MATERIALS AND METHODS

During a 1-year period, 240 water samples were collected from four university hospitals. All water samples were collected in sterile polystyrene bottles. A volume of 100ml of the samples passed through sterile 0.45-micrometer filters. The filters were placed directly on malt extract agar and incubated at 27°C for 3 to 7 days. Routine mycological techniques were applied to identification of grown fungi.

RESULTS

Out of 240 plates, 77.5% were positive for fungal growth. Twelve different genera were identified. Aspergillus (29.7%), Cladosporium (26.7%) and Penicillium (23.9%) were the most common isolated. Among Aspergillus species, A. flavus had the highest frequency. Highest colony counts were found in autumn. Aspergillus predominated in autumn, Cladosporium in winter and spring and Penicillium in summer.

CONCLUSION

The results of our study showed that hospital water should be considered as a potential reservoir of fungi particularly Aspergillus.

摘要

目的

本研究旨在评估伊朗萨里市大学医院自来水中的真菌菌群。

材料和方法

在为期一年的时间里,从 4 所大学医院采集了 240 份水样。所有水样均采集于无菌聚苯乙烯瓶中。将 100ml 体积的水样通过无菌 0.45 微米过滤器。将过滤器直接放置在麦芽提取物琼脂上,并在 27°C 下孵育 3 至 7 天。应用常规的真菌学技术对生长的真菌进行鉴定。

结果

在 240 个平板中,77.5%的平板有真菌生长。鉴定出了 12 个不同的属。最常见的分离菌是曲霉属(29.7%)、枝孢属(26.7%)和青霉属(23.9%)。在曲霉属中,黄曲霉的频率最高。秋季的菌落计数最高。秋季以曲霉为主,冬季和春季以枝孢属为主,夏季以青霉为主。

结论

我们的研究结果表明,医院用水应被视为真菌(尤其是曲霉属)的潜在储库。

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