Yonezawa S, Matsushita Y, Muramatsu H, Arita Y, Nakamura T, Muramatsu T, Sato E
Second Department of Pathology, Kagoshima University School of Medicine, Japan.
Acta Pathol Jpn. 1987 Aug;37(8):1249-61. doi: 10.1111/j.1440-1827.1987.tb00458.x.
Using the surgically extirpated specimens from 9 patients with colorectal carcinoma, fucosyltransferase activities in the carcinoma tissue and the normal mucosa were measured and were compared with the histochemical findings of glycoconjugates which were shown by staining with lectins reacting with blood group antigens and related substances. The fucosyltransferase activities of the carcinoma tissue were well correlated with the overall findings of lectin stainings after neuraminidase treatment. The more intense the carcinoma tissue was stained, the higher the fucosyltransferase activity was shown. However, there were marked differences in the fucosyltransferase activities by the portions measured, depending upon the relative amount of carcinoma tissue and interstitial tissue; in the invasive portion with less carcinoma tissue, the activity was generally low in comparison with that in the surface area where carcinoma tissue was rather abundant. Thus, the morphological and lectin histochemical finding are of paramount importance for the evaluation of glycosyltransferase activity in human colorectal carcinoma.
利用9例结直肠癌患者手术切除的标本，测定癌组织和正常黏膜中的岩藻糖基转移酶活性，并与通过与血型抗原及相关物质反应的凝集素染色所显示的糖缀合物的组织化学结果进行比较。癌组织的岩藻糖基转移酶活性与神经氨酸酶处理后凝集素染色的总体结果密切相关。癌组织染色越强烈，显示的岩藻糖基转移酶活性越高。然而，根据癌组织和间质组织的相对量，所测部位的岩藻糖基转移酶活性存在显著差异；在癌组织较少的浸润部位，与癌组织相当丰富的表面区域相比，活性通常较低。因此，形态学和凝集素组织化学结果对于评估人类结直肠癌中的糖基转移酶活性至关重要。
