岩藻糖基转移酶III和唾液酸化岩藻糖基-X（sialyl-Le(x)）的表达在培养的结肠癌细胞中相关，但在结肠癌组织中不相关。
Fucosyltransferase III and sialyl-Le(x) expression correlate in cultured colon carcinoma cells but not in colon carcinoma tissue.
作者信息
Hanski C, Klussmann E, Wang J, Böhm C, Ogorek D, Hanski M L, Krüger-Krasagakes S, Eberle J, Schmitt-Gräff A, Riecken E O
机构信息
Department of Gastroenterology, Klinikum Benjamin Franklin der Freien Universität, Berlin, FRG.
出版信息
Glycoconj J. 1996 Oct;13(5):727-33. doi: 10.1007/BF00702336.
The potential contribution of fucosyltransferases to the overexpression of sialyl-Le(x) antigen was investigated in the colon carcinoma cell line HT-29 and in human colon carcinoma tissue. In HT-29 cells as well as in normal or malignant colonic tissues Fuc-TIII, Fuc-TIV, Fuc-TVI but not Fuc-TV nor Fuc-TVII were detectable after RT-PCR. Sodium butyrate treatment of HT-29 cells increased (to about 200%) and DMSO treatment decreased (to about 20%) the expression of sialyl-Le(x). This modulation of sialyl-Le(x) was concomitant with the analogous increase/decrease of mRNA of Fuc-TIII but not Fuc-TIV. Fuc-TVI was not detectable by Northern blotting in HT-29 cells. In six human colon carcinomas which exhibited strong overexpression of sialyl-Le(x), the expression of Fuc-TIII-mRNA was the same or lower than in the corresponding normal colonic tissue. Thus Fuc-TIII expression may be affecting the expression of the sialyl-Le(x) moiety in HT-29 cells but not in human colon carcinoma tissue.
在结肠癌细胞系HT - 29和人结肠癌组织中研究了岩藻糖基转移酶对唾液酸化岩藻糖基路易斯x抗原（sialyl - Le(x)）过表达的潜在作用。经逆转录聚合酶链反应（RT - PCR）检测，在HT - 29细胞以及正常或恶性结肠组织中可检测到岩藻糖基转移酶III（Fuc - TIII）、岩藻糖基转移酶IV（Fuc - TIV）、岩藻糖基转移酶VI（Fuc - TVI），但未检测到岩藻糖基转移酶V（Fuc - TV）和岩藻糖基转移酶VII（Fuc - TVII）。丁酸钠处理HT - 29细胞可使唾液酸化岩藻糖基路易斯x抗原的表达增加（至约200%），而二甲基亚砜（DMSO）处理则使其表达降低（至约20%）。唾液酸化岩藻糖基路易斯x抗原的这种调节与Fuc - TIII的信使核糖核酸（mRNA）类似地增加/减少相伴，但Fuc - TIV并非如此。通过Northern印迹法在HT - 29细胞中未检测到Fuc - TVI。在6例唾液酸化岩藻糖基路易斯x抗原强烈过表达的人结肠癌中，Fuc - TIII - mRNA的表达与相应正常结肠组织相同或更低。因此，Fuc - TIII的表达可能影响HT - 29细胞中唾液酸化岩藻糖基路易斯x抗原部分的表达，但不影响人结肠癌组织中的表达。
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