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Evaluation of DNA extraction methods for Bacillus anthracis spores spiked to food and feed matrices at biosafety level 3 conditions.评价在生物安全 3 级条件下，添加到食品和饲料基质中的炭疽芽孢杆菌孢子的 DNA 提取方法。
Int J Food Microbiol. 2011 Nov 1;150(2-3):122-7. doi: 10.1016/j.ijfoodmicro.2011.07.023. Epub 2011 Jul 29.
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Interlaboratory evaluation of different extraction and real-time PCR methods for detection of Coxiella burnetii DNA in serum.不同提取和实时 PCR 方法检测血清中贝氏柯克斯体 DNA 的实验室间评估。
J Clin Microbiol. 2010 Nov;48(11):3923-7. doi: 10.1128/JCM.01006-10. Epub 2010 Sep 8.
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生物威胁因子自动化核酸样本提取设备的比较评估

Comparative assessment of automated nucleic acid sample extraction equipment for biothreat agents.

作者信息

Kalina Warren Vincent, Douglas Christina Elizabeth, Coyne Susan Rajnik, Minogue Timothy Devin

机构信息

Diagnostic Systems Division, United States Army Medical Research Institute of Infectious Disease, Fort Detrick, Maryland, USA.

出版信息

J Clin Microbiol. 2014 Apr;52(4):1232-4. doi: 10.1128/JCM.03453-13. Epub 2014 Jan 22.

DOI:10.1128/JCM.03453-13
PMID:24452173
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3993465/
Abstract

Magnetic beads offer superior impurity removal and nucleic acid selection over older extraction methods. The performances of nucleic acid extraction of biothreat agents in blood or buffer by easyMAG, MagNA Pure, EZ1 Advanced XL, and Nordiag Arrow were evaluated. All instruments showed excellent performance in blood; however, the easyMAG had the best precision and versatility.

摘要

与传统提取方法相比，磁珠在去除杂质和选择核酸方面具有更出色的表现。评估了easyMAG、MagNA Pure、EZ1 Advanced XL和Nordiag Arrow对血液或缓冲液中生物威胁因子核酸的提取性能。所有仪器在血液中的表现都很出色；然而，easyMAG具有最佳的精密度和通用性。