Tournois H, Killian J A, Urry D W, Bokking O R, de Gier J, de Kruijff B
Department of Biochemistry, University of Utrecht, The Netherlands.
Biochim Biophys Acta. 1987 Nov 27;905(1):222-6. doi: 10.1016/0005-2736(87)90026-5.
It is shown by 31P-NMR and small angle X-ray scattering that induction of an hexagonal HII phase in dioleoylphosphatidylcholine model membranes by external addition of gramicidin A' depends on the solvent which is used to solubilize the peptide. Addition of gramicidin from dimethylsulfoxide or trifluoroethanol solution leads to HII phase formation whereas addition of the peptide from ethanol does not. This solvent dependence is shown by circular dichroism to be correlated with the peptide conformation. The channel conformation appears to be responsible for HII phase formation by gramicidin.
31P-NMR和小角X射线散射表明，通过外部添加短杆菌肽A'在二油酰磷脂酰胆碱模型膜中诱导六方HII相取决于用于溶解该肽的溶剂。从二甲基亚砜或三氟乙醇溶液中添加短杆菌肽会导致HII相形成，而从乙醇中添加该肽则不会。圆二色性表明这种溶剂依赖性与肽的构象相关。通道构象似乎是短杆菌肽形成HII相的原因。
