Joshi-Navare Kasturi, Khanvilkar Poonam, Prabhune Asmita
Biochemical Sciences Division, National Chemical Laboratory, Homi Bhabha Road, Pune, Maharashtra 411008, India.
Biochem Res Int. 2013;2013:169797. doi: 10.1155/2013/169797. Epub 2013 Dec 21.
Sophorolipids (SLs) are glycolipidic biosurfactants suitable for various biological and physicochemical applications. The nonedible Jatropha oil has been checked as the alternative raw material for SL synthesis using C. bombicola (ATCC22214). This is useful towards lowering the SL production cost. Through optimization of fermentation parameters and use of resting cell method, the yield 15.25 g/L could be achieved for Jatropha oil derived SL (SLJO) with 1% v/v oil feeding. The synthesized SL displayed good surfactant property. It reduced the surface tension of distilled water from 70.7 mN/m to 33.5 mN/m with the Critical Micelle Concentration (CMC) value of 9.5 mg/L. Keeping the prospective use of the SL in mind, the physicochemical properties were checked along with emulsion stability under temperature, pH stress, and in hard water. Also antibacterial action and stain removal capability in comparison with commercial detergent was demonstrated. SLJO enhanced the detergent performance. Based on the results, it can be said that SLs have utility as fabric cleaner with advantageous properties such as skin friendly nature, antibacterial action, and biodegradability. Therefore SLs are potential green molecules to replace synthetic surfactants in detergents so as to reduce harm caused to environment through detergent usage.
槐糖脂(SLs)是一种糖脂类生物表面活性剂,适用于各种生物和物理化学应用。已对不可食用的麻风树油进行检测,以作为利用棉病囊霉(ATCC22214)合成SL的替代原料。这有助于降低SL的生产成本。通过优化发酵参数并采用静息细胞法,以1% v/v的油进料量,麻风树油衍生的SL(SLJO)产量可达15.25 g/L。合成的SL表现出良好的表面活性剂性能。它将蒸馏水的表面张力从70.7 mN/m降低到33.5 mN/m,临界胶束浓度(CMC)值为9.5 mg/L。考虑到SL的潜在用途,对其物理化学性质以及在温度、pH值胁迫和硬水中的乳液稳定性进行了检测。还展示了与市售洗涤剂相比的抗菌作用和去污能力。SLJO提高了洗涤剂性能。基于这些结果,可以说SLs作为织物清洁剂具有实用性,具有诸如亲肤性、抗菌作用和生物降解性等优势特性。因此,SLs是替代洗涤剂中合成表面活性剂的潜在绿色分子,以减少洗涤剂使用对环境造成的危害。