Leyton Allison, Araya Michael, Tala Fadia, Flores Liset, Lienqueo María Elena, Shene Carolina
Center for Biotechnology and Bioengineering (CeBiB), Center of Food Biotechnology and Bioseparations, BIOREN and Department of Chemical Engineering, Universidad de La Frontera, Francisco Salazar 01145, Temuco 4780000, Chile.
Centro de Investigación y Desarrollo Tecnológico de Algas y otros Recursos Biológicos (CIDTA), Facultad de Ciencias Marinas, Universidad Católica del Norte, Coquimbo 17811421, Chile.
Molecules. 2021 Apr 18;26(8):2355. doi: 10.3390/molecules26082355.
Seaweed processing generates liquid fraction residual that could be used as a low-cost nutrient source for microbial production of metabolites. The strain is able to produce antimicrobial compounds known as sophorolipids. Our aim was to evaluate sophorolipid production, with antibacterial activity, by marine using liquid fraction residual (LFR) from the brown seaweed as the nutrient source. LFR having a composition of 32% / carbohydrate, 1% / lipids, 15% / protein and 52% / ash. The best culture condition for sophorolipid production was LFR 40% /, without yeast extract, artificial seawater 80% / at 15 °C by 3 growth days, with the antibacterial activity of 24.4 ± 3.1 % on and 21.1 ± 3.8 % on It was possible to identify mono-acetylated acidic and methyl ester acidic sophorolipid. These compounds possess potential as pathogen controllers for application in the food industry.
海藻加工会产生液体部分残余物,其可用作微生物生产代谢物的低成本营养源。该菌株能够产生被称为槐糖脂的抗菌化合物。我们的目标是评估利用褐藻的液体部分残余物(LFR)作为营养源,通过海洋生物生产具有抗菌活性的槐糖脂。LFR的组成包括32%的碳水化合物、1%的脂质、15%的蛋白质和52%的灰分。生产槐糖脂的最佳培养条件是40%的LFR、无酵母提取物、80%的人工海水、在15℃下培养3天,对金黄色葡萄球菌的抗菌活性为24.4±3.1%,对大肠杆菌的抗菌活性为21.1±3.8%。有可能鉴定出单乙酰化酸性和甲酯酸性槐糖脂。这些化合物具有作为病原体控制剂应用于食品工业的潜力。