LC-NMR 技术在天然提取物中植物甾醇分析中的应用。
LC-NMR Technique in the Analysis of Phytosterols in Natural Extracts.
机构信息
Institute of Chemical Process Fundamentals of the ASCR, v.v.i., Rozvojová 2/135, 16502 Prague 6, Czech Republic.
Institute of Organic Chemistry and Biochemistry of the ASCR, v.v.i., Flemingovo Náměstí 2, 16610 Prague 6, Czech Republic.
出版信息
J Anal Methods Chem. 2013;2013:526818. doi: 10.1155/2013/526818. Epub 2013 Dec 23.
The ability of LC-NMR to detect simultaneously free and conjugated phytosterols in natural extracts was tested. The advantages and disadvantages of a gradient HPLC-NMR method were compared to the fast composition screening using SEC-NMR method. Fractions of free and conjugated phytosterols were isolated and analyzed by isocratic HPLC-NMR methods. The results of qualitative and quantitative analyses were in a good agreement with the literature data.
利用 LC-NMR 同时检测天然提取物中的游离态和结合态植物甾醇的能力已经过测试。梯度 HPLC-NMR 方法与 SEC-NMR 方法的快速组成筛选进行了比较。采用等度 HPLC-NMR 方法对游离态和结合态植物甾醇的馏分进行了分离和分析。定性和定量分析的结果与文献数据吻合良好。
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