中国蝎子 Buthusmartensii Karsch 的两种生物活性化合物。

Two bioactive compounds from the Chinese scorpion Buthusmartensii Karsch.

机构信息

a School of Chemical Engineering and Pharmaceutics, Henan University of Science and Technology , Luoyang , Henan 471023 , P.R. China.

出版信息

Nat Prod Res. 2014;28(10):698-703. doi: 10.1080/14786419.2013.873433. Epub 2014 Jan 23.

DOI:10.1080/14786419.2013.873433

PMID:24456483

Abstract

Two compounds, 3β-acetoxyl,2,14,22-trihydroxy,19-hydroxymethyl,9α,5β,14β-card-20(22)enolide (1) and 1,2,3,4-tetrahydro-6-hydroxy1-5-pyrimidinecarbox-aldehyde (2), were isolated from arthropods in scorpion, Buthus martensii Karsch, by medium pressure liquid chromatography with silica gel as stationary phase and RP-HPLC analysis technology. The structures were elucidated on the basis of NMR spectroscopic analysis and HR-ESI-MS determination. It was found that (1) is a novel compound and both compounds showed inhibitory activities against the Gram-positive bacteria Bacillus subtilis (minimum inhibitory concentrations = 15 μg/mL), with diameter of inhibition zone at ϕ = 6.0 mm (compound 1) and ϕ = 9.0 mm (compound 2), respectively.

摘要

两种化合物，3β-乙酰氧基，2,14,22-三羟基，19-羟甲基，9α,5β,14β-卡-20(22)烯内酯 (1) 和 1,2,3,4-四氢-6-羟基-1-嘧啶羧酸醛 (2)，从节肢动物蝎子（Buthus martensii Karsch）中通过中压液相色谱用硅胶作为固定相和 RP-HPLC 分析技术分离得到。根据 NMR 光谱分析和 HR-ESI-MS 测定确定了它们的结构。发现（1）是一种新型化合物，两种化合物都显示出对革兰氏阳性菌枯草芽孢杆菌（最小抑菌浓度 = 15 μg/mL）的抑制活性，抑菌圈直径分别为 ϕ = 6.0 毫米（化合物 1）和 ϕ = 9.0 毫米（化合物 2）。