Böttinger H, Reuner K H, Aktories K
Rudolf-Buchheim-Institut für Pharmakologie, Giessen, BRD.
Int Arch Allergy Appl Immunol. 1987;84(4):380-4. doi: 10.1159/000234453.
The effects of botulinum C2 toxin, which ADP-ribosylates actin, were studied on the stimulated histamine release of rat peritoneal mast cells. Treatment of mast cells with botulinum C2 toxin for 4 h inhibited histamine release stimulated by compound 48/80 or MCD-peptide maximally by about 50%. A half-maximal and maximal inhibition occurred at about 30 and 450 ng/ml botulinum C2 toxin. At similar concentrations the toxin ADP-ribosylated actin in intact mast cells. Botulinum C2 toxin largely impaired the histamine release stimulated by 12-O-tetradecanoylphorbol-13-acetate but not by the ionophore A23187. The data indicate that botulinum C2 toxin partially inhibits the stimulated histamine release and suggest that actin is involved in the stimulus-secretion coupling in mast cells.
研究了可使肌动蛋白进行 ADP 核糖基化的肉毒杆菌 C2 毒素对大鼠腹膜肥大细胞组胺释放的影响。用肉毒杆菌 C2 毒素处理肥大细胞 4 小时，可最大程度地抑制由化合物 48/80 或 MCD 肽刺激引起的组胺释放，抑制率约为 50%。在约 30 和 450 ng/ml 的肉毒杆菌 C2 毒素浓度下分别出现半最大抑制和最大抑制。在相似浓度下，该毒素可使完整肥大细胞中的肌动蛋白进行 ADP 核糖基化。肉毒杆菌 C2 毒素在很大程度上损害了由 12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯刺激引起的组胺释放，但对离子载体 A23187 刺激引起的组胺释放没有影响。数据表明肉毒杆菌 C2 毒素部分抑制刺激引起的组胺释放，并提示肌动蛋白参与肥大细胞的刺激 - 分泌偶联过程。
