Shen Yi, Redmond Sharon L, Papadimitriou John M, Teh Bing M, Yan Sheng, Wang Yan, Atlas Marcus D, Marano Robert J, Zheng Minghao, Dilley Rodney J
Ear Sciences Centre, School of Surgery, The University of Western Australia, Nedlands 6009, Western Australia, Australia. Ear Science Institute Australia, Subiaco 6008, Western Australia, Australia. Department of Otolaryngology, Head & Neck, Ningbo Lihuili Hospital (Ningbo Medical Centre), Ningbo 315041, Zhejiang, People's Republic of China.
Biomed Mater. 2014 Feb;9(1):015015. doi: 10.1088/1748-6041/9/1/015015. Epub 2014 Jan 23.
Recent experimental studies have shown the suitability of silk fibroin scaffold (SFS) and porcine-derived acellular collagen I/III scaffold (ACS) as onlay graft materials for tympanic membrane perforation repair. The aims of this study were to further characterize and evaluate the in vivo biocompatibility of SFS and ACS compared with commonly used materials such as Gelfoam and paper in a rat model. The scaffolds were implanted in subcutaneous (SC) tissue and middle ear (ME) cavity followed by histological and otoscopic evaluation for up to 26 weeks. Our results revealed that SFS and ACS were well tolerated and compatible in rat SC and ME tissues throughout the study. The tissue response adjacent to the implants evaluated by histology and otoscopy showed SFS and ACS to have a milder tissue response with minimal inflammation compared to that of paper. Gelfoam gave similar results to SFS and ACS after SC implantation, but it was found to be associated with pronounced fibrosis and osteoneogenesis after ME implantation. It is concluded that SFS and ACS both were biocompatible and could serve as potential alternative scaffolds for tissue engineering in the ear.
最近的实验研究表明，丝素蛋白支架（SFS）和猪源脱细胞I/III型胶原支架（ACS）作为鼓膜穿孔修复的外置移植材料具有适用性。本研究的目的是在大鼠模型中，进一步表征和评估SFS和ACS与常用材料（如明胶海绵和纸片）相比的体内生物相容性。将这些支架植入皮下（SC）组织和中耳（ME）腔，随后进行长达26周的组织学和耳镜评估。我们的结果显示，在整个研究过程中，SFS和ACS在大鼠的SC和ME组织中耐受性良好且具有相容性。通过组织学和耳镜评估植入物附近的组织反应，结果显示与纸片相比，SFS和ACS的组织反应更轻微，炎症最少。明胶海绵在SC植入后与SFS和ACS的结果相似，但在ME植入后发现它与明显的纤维化和骨生成有关。结论是，SFS和ACS均具有生物相容性，可作为耳部组织工程的潜在替代支架。
