含T辅助细胞1表位的合成肽疫苗与CpG寡脱氧核苷酸联合对猫传染性腹膜炎病毒感染猫的保护效力评估
Evaluation of protective efficacy of the synthetic peptide vaccine containing the T-helper 1 epitope with CpG oligodeoxynucleotide against feline infectious peritonitis virus infection in cats.
作者信息
Takano Tomomi, Tomizawa Keisuke, Morioka Hiroyuki, Doki Tomoyoshi, Hohdatsu Tsutomu
机构信息
Laboratory of Veterinary Infectious Disease, School of Veterinary Medicine, Kitasato University, Towada, Japan.
出版信息
Antivir Ther. 2014;19(7):645-50. doi: 10.3851/IMP2735. Epub 2014 Jan 23.
BACKGROUND
Feline infectious peritonitis (FIP) is a feline coronavirus-induced fatal disease in domestic and wild cats. Cellular immunity is considered to play an important role in the prevention of FIP. Thus, induction of the cellular immune response is essential in vaccines against FIP virus (FIPV) infection.
METHODS
We immunized cats with peptides containing T-helper (Th)1 epitopes derived from the nucleocapsid (N) protein of the type I FIPV KU-2 strain (NP7 and NP8) with feline CpG-oligodeoxynucleotides (fCpG-ODNs) as a vaccine adjuvant.
RESULTS
Prevention against type II FIPV 79-1146 strain-induced FIP was slightly better in specific pathogen-free cats treated with NP7 and NP8 with fCpG-ODNs. However, immune tolerance was suggested to be induced by the high dose and frequency of NP7 and NP8 with fCpG-ODNs.
CONCLUSIONS
Further investigations on the combination and concentrations of the peptides and fCpG-ODNs, dose, frequency and route of administration are needed.
背景
猫传染性腹膜炎（FIP）是一种由猫冠状病毒引起的家猫和野猫的致命疾病。细胞免疫被认为在预防FIP中起重要作用。因此，诱导细胞免疫反应在针对猫传染性腹膜炎病毒（FIPV）感染的疫苗中至关重要。
方法
我们用含有来自I型FIPV KU-2株核衣壳（N）蛋白的T辅助（Th）1表位的肽（NP7和NP8）免疫猫，并将猫CpG寡脱氧核苷酸（fCpG-ODN）作为疫苗佐剂。
结果
在用NP7和NP8与fCpG-ODN处理的无特定病原体猫中，对II型FIPV 79-1146株诱导的FIP的预防效果略好。然而，提示NP7和NP8与fCpG-ODN的高剂量和高频率会诱导免疫耐受。
结论
需要对肽和fCpG-ODN的组合及浓度、剂量、给药频率和途径进行进一步研究。
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