Agricultural Research Council Unit of Developmental Botany, 181A Huntingdon Road, CB3 ODY, Cambridge, UK.
Planta. 1974 Sep;117(3):259-68. doi: 10.1007/BF00388399.
Ribosome preparations from several species of flowering plant were found to contain ribonuclease. It was predominantly latent in ionic conditions which maintained the integrity of the ribosomes but was activated by agents which disrupted ribosome structure by chelating their divalent cations. The ribonuclease was not a structural component of the ribosomes but was preferentially adsorbed by them upon disruption of cells. Adsorption took place onto both large and small ribosomal subunits, and onto monosomes and polysomes. These results are discussed in relation to possible mechanisms for the breakdown of ribosomal RNA in vivo.
从几种开花植物的核糖体制剂中发现含有核糖核酸酶。它主要是潜伏在离子条件下，这种离子条件维持核糖体的完整性，但被通过螯合其二价阳离子破坏核糖体结构的试剂激活。核糖核酸酶不是核糖体的结构组成部分，但在细胞破裂时优先被核糖体吸附。吸附发生在大、小核糖体亚基上，以及在单核糖体和多核糖体上。这些结果与体内核糖体 RNA 降解的可能机制有关进行了讨论。
