Carnegie Institution of Washington, 94305, Stanford, CA, USA.
Photosynth Res. 1983 Dec;4(4):375-83. doi: 10.1007/BF00054145.
The three major chlorophyll-proteins of spinach chloroplasts were solubilized with digitonin and isolated by electrophoresis with deoxycholate. The gel bands were identified from their absorption and fluorescence spectra measured at 77 K. The slowest moving band was a Photosystem I complex (CPI); the second, a Photosystem II complex (Cpa); and the third, a chlorophyll a-b, antenna complex (LHCP). When absorption spectra (630-730 nm) of the bands were added in the proportions found in the gel, the sum closely matched the absorption of the chloroplasts both before and after solubilization. Thus these spectra represent the native absorption of the major antenna chlorophyll-proteins of green plants. Each of these spectra was resolved with a computer assisted, curve-fitting program into 8 mixed Gaussian-Lorentzian shaped components. The major, Chl a components in the 3 fractions were different both in peak positions and bandwidths. This result suggests that each chlorophyll-protein has its own unique set of chlorophyll a spectral forms or components.
菠菜叶绿体中的三种主要叶绿素蛋白用去氧胆酸钠溶解，并通过胆酸盐电泳分离。从 77 K 下测量的吸收和荧光光谱鉴定凝胶带。移动最慢的带是光系统 I 复合物（CPI）；第二个是光系统 II 复合物（Cpa）；第三个是叶绿素 a-b，天线复合物（LHCP）。当将凝胶中发现的比例的带的吸收光谱（630-730nm）相加时，总和与溶解前后的叶绿体的吸收非常吻合。因此，这些光谱代表了绿色植物主要天线叶绿素蛋白的天然吸收。使用计算机辅助的曲线拟合程序将每个这些光谱分解为 8 个混合高斯-洛伦兹形状的分量。这三个部分中的主要 Chl a 成分在峰位置和带宽方面都不同。这一结果表明，每种叶绿素蛋白都有其独特的叶绿素 a 光谱形式或成分。
