Murray K, Bruce S A, Wingfield P, van Eerd P, de Reus A, Schellekens H
Department of Molecular Biology, University of Edinburgh, Scotland.
J Med Virol. 1987 Oct;23(2):101-7. doi: 10.1002/jmv.1890230202.
Preparations of hepatitis B virus (HBV) core antigen (HBcAg) synthesised in Escherichia coli have been shown previously to confer partial immunity against infection by the virus [Murray, Bruce, Hinnen, Wingfield, van Eerd, de Reus, and Schellekens: EMBO Journal 3:645-650, 1984]. In a further experiment reported here, immunisation of chimpanzees with a similar preparation of HBcAg that had been treated with sodium dodecyl sulphate in order to expose e antigen epitopes was found to protect one animal completely and another quite substantially upon challenge with the virus. The results are used to support the argument for trials in humans of a vaccine against HBV based upon or containing HBcAg and its e antigen derivative, and in discussion of a more general role for internal antigens in generating immunity against viral infection.
先前已表明，在大肠杆菌中合成的乙型肝炎病毒（HBV）核心抗原（HBcAg）制剂可赋予针对该病毒感染的部分免疫力[默里、布鲁斯、欣嫩、温菲尔德、范·埃尔德、德·鲁斯和谢勒肯斯：《欧洲分子生物学组织杂志》3:645 - 650，1984年]。在此报道的进一步实验中，用一种经过十二烷基硫酸钠处理以暴露e抗原表位的类似HBcAg制剂对黑猩猩进行免疫，结果发现，在用该病毒攻击时，一只动物得到了完全保护，另一只动物也得到了相当程度的保护。这些结果被用于支持基于HBcAg及其e抗原衍生物或包含它们的乙肝疫苗进行人体试验的论点，并用于讨论内部抗原在产生针对病毒感染的免疫力方面更广泛的作用。
