Department of Pharmacy and Biotechnology, University of Bologna, Bologna, Italy.
Analyst. 2014 Mar 7;139(5):1201-7. doi: 10.1039/c3an01737k. Epub 2014 Jan 24.
The present study investigated the analytical capabilities of a new fluorescent chemosensor, named DCHQ5, a phenyl derivative belonging to the family of diaza-crown-hydroxyquinolines, for the quantitative assessment of total intracellular Mg content. The results obtained were compared to the analytical performances of DCHQ1 - the parent probe of the series which so far was the only suitable species for the quantitative assessment of the intracellular total magnesium and showed comparable results to atomic absorption spectroscopy. Different protocols were tested in several cell lines both by flow cytometry and by steady state fluorescence spectroscopy assays. The results obtained support the possibility to use DCHQ5 to accurately quantify the intracellular total Mg in much smaller samples than DCHQ1, also displaying an increased stable intracellular staining. These features, combined with the high fluorescence enhancement upon cation binding, and the possibility to be excited both in the UV and visible region, make DCHQ5 a valuable and versatile analytical tool for Mg assessment in biological samples.
本研究考察了一种新的荧光化学传感器 DCHQ5 的分析能力，DCHQ5 是一种苯衍生的二氮杂冠羟基喹啉类化合物，用于定量评估细胞内总镁含量。将获得的结果与 DCHQ1（该系列的母体探针）的分析性能进行了比较，到目前为止，DCHQ1 是唯一适合定量评估细胞内总镁的物质，并且与原子吸收光谱法具有可比的结果。通过流式细胞术和稳态荧光光谱法测定，在几种细胞系中测试了不同的方案。获得的结果支持使用 DCHQ5 来准确地定量比 DCHQ1 小得多的细胞内总镁的可能性，并且显示出增强的稳定的细胞内染色。这些特征，结合阳离子结合时的高荧光增强，以及在紫外和可见光区域都可以激发的可能性，使得 DCHQ5 成为生物样品中镁评估的一种有价值和多功能的分析工具。
