Department of Applied Microbiology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Warsaw
Department of Mycology, Chair of Microbiology, Collegium Medicum, Jagiellonian University, Cracow.
Pol J Microbiol. 2013;62(3):303-6.
The genus Scopulariopsis contains over 30 species of mitosporic moulds, which although usually saprophytic may also act as opportunistic pathogens in humans. They have mainly been associated with onychomycosis, and only sporadically reported as a cause of deep tissue infections or systemic disease. Identification of Scopulariopsis species still largely relies on phenotype-based methods. There is a need for a molecular diagnostic approach, that would allow to reliably discriminate between different Scopulariopsis species. The aim of this study was to apply sequence analysis of partial 28S rRNA gene for species identification of Scopulariopsis clinical isolates. Although the method employed did reveal some genetic polymorphism among Scopulariopsis isolates tested, it was not enough for species delineation. For this to be achieved, other genetic loci, within and beyond the rDNA operon, need to be investigated.
帚霉属包含超过 30 种的半知菌，虽然它们通常是腐生的，但也可能成为人类的机会性病原体。它们主要与甲真菌病有关，只是偶尔有报道作为深部组织感染或全身性疾病的原因。帚霉属种的鉴定仍然主要依赖于表型为基础的方法。需要一种分子诊断方法，能够可靠地区分不同的帚霉属种。本研究旨在应用部分 28S rRNA 基因序列分析对帚霉属临床分离株进行种属鉴定。尽管所采用的方法确实揭示了测试的帚霉属分离株之间存在一些遗传多态性，但不足以进行物种划分。要做到这一点，需要研究 rDNA 操纵子内外的其他遗传基因座。
