Jagielski Tomasz, Sandoval-Denis Marcelo, Yu Jin, Yao Limin, Bakuła Zofia, Kalita Joanna, Skóra Magdalena, Krzyściak Paweł, de Hoog G Sybren, Guarro Josep, Gené Josepa
Department of Applied Microbiology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Warsaw, Poland.
Unitat de Micologia, Facultat de Medicina i Ciències de la Salut, IISPV, Universitat Rovira i Virgili, Reus, Spain.
Fungal Biol. 2016 Apr;120(4):586-602. doi: 10.1016/j.funbio.2016.01.014. Epub 2016 Feb 3.
The taxonomy of scopulariopsis-like fungi, comprising numerous human opportunistic species, has recently been reassessed with delineation of the genera Microascus, Pithoascus, Pseudoscopulariopsis, and Scopulariopsis, using morphological data and multilocus sequence analysis based on four loci (ITS, LSU, EF-1α, and TUB). In this study, the same genetic markers were used to investigate a set of clinical and environmental isolates, morphologically identified as Microascus and Scopulariopsis spp. The ingroups of the concatenated phylogenetic tree resolved 41 species clades, with isolates distributed in four main lineages corresponding to the genera Microascus, Pithoascus, Scopulariopsis, and newly established genus Fuscoannellis, typified by Scopulariopsis carbonaria. The new species Microascus chinensis, Microascus onychoides, Microascus pseudolongirostris, Pithoascus lunatus, and Scopulariopsis macurae were described. Microascus trigonosporus var. terreus and Scopulariopsis alboflavescens were found different from M. trigonosporus and Scopulariopsis brevicaulis, respectively. All the species identified in the study, except Fuscoannellis carbonaria and S. macurae, originated from clinical samples, suggesting their potential role in human disease. The use of a four marker combination was demonstrated an efficient and reliable approach to infer phylogenetic relationships among the scopulariopsis-like fungi. Yet, the only genetic marker able to discriminate all species was EF-1α, therefore proposed as a secondary barcode for the identification of these fungi.
拟帚霉属真菌包含众多人类机会致病菌，最近利用形态学数据以及基于四个基因座（ITS、LSU、EF-1α和TUB）的多位点序列分析，对其分类进行了重新评估，划分出了微小曲霉属、皮托曲霉属、假帚霉属和帚霉属。在本研究中，使用相同的遗传标记对一组形态学上鉴定为微小曲霉属和帚霉属的临床和环境分离株进行了研究。串联系统发育树的内类群解析出41个物种分支，分离株分布在四个主要谱系中，分别对应微小曲霉属、皮托曲霉属、帚霉属以及新建立的Fuscoannellis属，该属以炭色帚霉为模式种。描述了新物种中华微小曲霉、甲癣微小曲霉、假长喙微小曲霉、新月皮托曲霉和黄斑帚霉。发现三角孢微小曲霉土生变种和微黄帚霉分别与三角孢微小曲霉和短帚霉不同。本研究中鉴定的所有物种，除了炭色Fuscoannellis和黄斑帚霉外，均源自临床样本，表明它们在人类疾病中可能发挥的作用。使用四个标记组合被证明是推断拟帚霉属真菌系统发育关系的一种有效且可靠的方法。然而，唯一能够区分所有物种的遗传标记是EF-1α，因此建议将其作为这些真菌鉴定的二级条形码。
