Sandoval-Denis Marcelo, Sutton Deanna A, Fothergill Annette W, Cano-Lira Josep, Gené Josepa, Decock C A, de Hoog G S, Guarro Josep
Unitat de Microbiologia, Facultat de Medicina i Ciències de la Salut, IISPV, Universitat Rovira i Virgili, Reus, Spain.
J Clin Microbiol. 2013 Dec;51(12):3937-43. doi: 10.1128/JCM.01927-13. Epub 2013 Sep 11.
Ninety-nine isolates of clinical origin, tentatively identified as Scopulariopsis or Microascus, were morphologically and molecularly characterized by a combined analysis of the D1/D2 domains of the 28S rRNA gene and a fragment of the elongation factor 1-α gene (EF1-α) sequences. The most prevalent species was Scopulariopsis brevicaulis (49.4%), followed by Scopulariopsis gracilis (14.4%), Scopulariopsis brumptii (7.2%), Microascus cinereus (5.2%), the Scopulariopsis candida species complex (3.1%), and Microascus cirrosus (2.1%). The most common anatomic sites of isolation were the respiratory tract (61.6%), superficial tissue (19.2%), and deep tissue or fluid samples (19.2%). The antifungal susceptibilities of the isolates to eight drugs were tested in vitro, with all the drugs generally showing poor activity.
99株临床分离株，初步鉴定为帚霉属或小囊菌属，通过对28S rRNA基因的D1/D2结构域和延伸因子1-α基因（EF1-α）序列片段进行联合分析，从形态学和分子水平上进行了特征鉴定。最常见的菌种是短帚霉（49.4%），其次是纤细帚霉（14.4%）、布鲁姆帚霉（7.2%）、灰小囊菌（5.2%）、念珠帚霉复合种（3.1%）和卷枝小囊菌（2.1%）。最常见的分离解剖部位是呼吸道（61.6%）、浅表组织（19.2%）和深部组织或体液样本（19.2%）。对这些分离株进行了8种药物的体外抗真菌药敏试验，结果显示所有药物的活性普遍较差。
