Carpenter S, Evans L H, Sevoian M, Chesebro B
Laboratory of Persistent Viral Diseases, National Institute of Allergy and Infectious Diseases, Rocky Mountain Laboratories, Hamilton, Montana 59840.
J Virol. 1987 Dec;61(12):3783-9. doi: 10.1128/JVI.61.12.3783-3789.1987.
Equine infectious anemia virus was isolated from peripheral blood leukocytes collected during two early febrile cycles of an experimentally infected horse. RNase T1-resistant oligonucleotide fingerprint analyses indicated that the nucleotide sequences of the isolates differed by approximately 0.25% and that the differences appeared randomly distributed throughout the genome. Serum collected in the interval between virus isolations was able to distinguish the isolates by membrane immunofluorescence on live cells. However, no neutralizing antibody was detected in the interval between virus isolations. In fact, multiple clinical cycles occurred before the development of a neutralizing antibody response, indicating that viral neutralization might not be the mechanism for selection of antigenic variants. The ability of early immune sera to recognize variant specific antigens on the surface of infected cells suggested that immune selection occurs through recognition and elimination of certain virus-infected cells. Alternately, the random distribution of the genomic differences observed between the two isolates may indicate that equine infectious anemia virus variants emerge as a result of nonimmunological selection processes.
从一匹实验感染马的两个早期发热周期采集的外周血白细胞中分离出了马传染性贫血病毒。核糖核酸酶T1抗性寡核苷酸指纹分析表明，分离株的核苷酸序列差异约为0.25%，且这些差异似乎随机分布于整个基因组。在病毒分离间隔期采集的血清能够通过活细胞上的膜免疫荧光区分分离株。然而，在病毒分离间隔期未检测到中和抗体。事实上，在中和抗体反应出现之前发生了多个临床周期，这表明病毒中和可能不是抗原变异体选择的机制。早期免疫血清识别感染细胞表面变异体特异性抗原的能力表明，免疫选择是通过识别和清除某些病毒感染细胞来进行的。或者，在两个分离株之间观察到的基因组差异的随机分布可能表明，马传染性贫血病毒变异体是由于非免疫选择过程而出现的。
