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采用荧光 PARAFAC 分析和色谱多激发/发射扫描技术表征城市废水中的溶解有机物:一项比较研究。

Characterization of dissolved organic matter in municipal wastewater using fluorescence PARAFAC analysis and chromatography multi-excitation/emission scan: a comparative study.

机构信息

State Key Laboratory of Pollution Control and Resources Reuse, Collaborative Innovation Center for Advanced Water Pollution Control Technology and Equipment, School of the Environment, Nanjing University , Nanjing, 210023, China.

出版信息

Environ Sci Technol. 2014;48(5):2603-9. doi: 10.1021/es404624q. Epub 2014 Feb 10.

Abstract

Dissolved organic matter (DOM) in municipal wastewater was mainly characterized using high-performance liquid chromatography (HPLC) and size exclusion chromatography (HPSEC) with multi-excitation/emission fluorescence scan. Meanwhile, fluorescence excitation-emission-matrix combined with parallel factor analysis (EEM-PARAFAC) was also applied. Compared with chromatography fluorescence fingerprints, the EEM-PARAFAC model could not reflect the variety of DOM species with similar fluorescence but different physicochemical properties. The chromatography results showed that the protein-like species were variable among different municipal wastewater treatment plants, some of which are in combination with humic-like species; while there were two major humic-like species fractionated by hydrophilicity and molecular weight (MW), which are also the major contributors to UV absorbance at 254 nm. It was also identified that the relatively hydrophilic humic fractions were slightly larger than the relatively hydrophobic humic fractions. In all the investigated wastewater treatment plants, the relatively hydrophilic and larger MW humic fraction mainly contributed to the fluorescence intensity of humic-like EEM-PARAFAC components. As well as facilitating interpretations of EEM-PARAFAC components, the HPLC/HPSEC fluorescence fingerprints also contributed to a better understanding of fluorescent DOM species in municipal wastewater.

摘要

采用高效液相色谱 (HPLC) 和体积排阻色谱 (HPSEC) 联用多激发/发射荧光扫描技术对城市废水中的溶解有机 matter (DOM) 进行了主要特性研究。同时,还应用了荧光激发-发射矩阵结合平行因子分析 (EEM-PARAFAC)。与色谱荧光指纹图谱相比,EEM-PARAFAC 模型无法反映具有相似荧光但具有不同物理化学性质的 DOM 物质的变化。色谱结果表明,不同城市污水处理厂的蛋白类物质变化较大,其中一些与类腐殖质物质结合;而亲水性和分子量 (MW) 分离的两种主要类腐殖质物质也是 254nm 紫外吸光度的主要贡献者。还确定了相对亲水的腐殖质部分比相对疏水的腐殖质部分稍大。在所研究的所有污水处理厂中,相对亲水和较大 MW 的腐殖质部分主要贡献了类腐殖质 EEM-PARAFAC 成分的荧光强度。HPLC/HPSEC 荧光指纹图谱不仅有助于解释 EEM-PARAFAC 成分,还有助于更好地理解城市废水中的荧光 DOM 物质。

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