Wu Xueshen, Wang Chao, Wang Depeng, Tawfik Ahmed, Xu Ronghua, Yu Zhong, Meng Fangang
School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510006, China.
Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Guangzhou 510006, China.
Eng Microbiol. 2023 Dec 15;4(1):100136. doi: 10.1016/j.engmic.2023.100136. eCollection 2024 Mar.
In this study, a combined system consisting of an anaerobic membrane bioreactor (AnMBR) and flow-through biofilm reactor/CANON (FTBR/CANON) was developed to simultaneously remove carbon and nitrogen from synthetic livestock wastewater. The average removal efficiencies of total nitrogen (TN) were 64.2 and 76.4% with influent ammonium (NH -N) concentrations of approximately 200 and 500 mg/L, respectively. The COD removal efficiencies were higher than 98.0% during the entire operation. Mass balance analysis showed that COD and TN were mainly removed by the AnMBR and FTBR/CANON, respectively. The anammox process was the main nitrogen removal pathway in the combined system, with a contribution of over 80%. High functional bacterial activity was observed in the combined system. Particularly, an increase in the NH -N concentration considerably improved the anammox activity of the biofilm in the FTBR/CANON. 16S rRNA high-throughput sequencing revealed that , and were the dominant methanogens in the AnMBR granular sludge. In the CANON biofilm, and Kuenenia were identified as aerobic and anaerobic ammonium-oxidizing bacteria, respectively. In summary, this study proposes a combined AnMBR and FTBR/CANON process targeting COD and nitrogen removal, and provides a potential alternative for treating high-strength wastewater.
在本研究中，开发了一种由厌氧膜生物反应器（AnMBR）和流通式生物膜反应器/全程自养脱氮工艺（FTBR/CANON）组成的联合系统，用于同时去除合成畜禽废水中的碳和氮。进水铵（NH₄⁺-N）浓度分别约为200和500 mg/L时，总氮（TN）的平均去除效率分别为64.2%和76.4%。在整个运行过程中，化学需氧量（COD）去除效率高于98.0%。质量平衡分析表明，COD和TN主要分别由AnMBR和FTBR/CANON去除。厌氧氨氧化过程是联合系统中主要的脱氮途径，贡献率超过80%。在联合系统中观察到较高的功能细菌活性。特别是，NH₄⁺-N浓度的增加显著提高了FTBR/CANON中生物膜的厌氧氨氧化活性。16S rRNA高通量测序显示，甲烷杆菌属、甲烷鬃毛菌属和甲烷八叠球菌属是AnMBR颗粒污泥中的优势产甲烷菌。在全程自养脱氮生物膜中，亚硝化单胞菌属和好氧厌氧氨氧化菌库氏菌分别被鉴定为好氧和厌氧氨氧化细菌。总之，本研究提出了一种针对COD和氮去除的AnMBR和FTBR/CANON联合工艺，为处理高强度废水提供了一种潜在的替代方案。
